EMBO Practical Course NMR

Structure, dynamics and function of biomacromolecules by solution NMR

Biozentrum Basel, 20 – 27 July 2013

The intention of the course is to provide practical training in important aspects of structure determination by NMR. The emphasis will be on setting up experiments on the spectrometer (optimizing parameters, pulse programs), on processing and analyzing the NMR data and on structure calculations in a hands-on fashion. It is impossible to also teach all the necessary theoretical background of modern NMR experiments in a few days. Therefore, the course is aimed at PhD students and postdocs who have some working knowledge of NMR theory, i.e. product operator formalism, and who understand the basic principles of NMR pulse sequences. Possible candidates should have the level of knowledge of students that have participated in a past "Advanced NMR" EMBO lecture course on NMR theory organized by Boelens, Oschkinat and Griesinger.

Topics

  • Pulse sequences for assignment and structural information: theory, practical set-up and use
  • Multidimensional data processing
  • Resonance assignment
  • Residual dipolar couplings: theory, experiments, data analysis
  • Paramagnetic NMR: theory, experiments, data analysis
  • Relaxation: theory, experiments, data analysis
  • Structure calculation and evaluation