Antimicrobial resistance is a burgeoning global health threat. Understanding the genetic changes and evolutionary trajectories leading to elevated antibiotic resistance can inform novel strategies for combating drug resistance. In this seminar, I will first introduce a novel automated microbial selection device, the “morbidostat”, which we developed for studying evolution of antibiotic resistance. Following that, I will summarize our recent work where we show that antibiotic resistance evolves through an extremely rugged adaptive landscape with direct and indirect paths leading to distinct resistance peaks. I will summarize what we learned from evolving antibiotic-resistant bacteria in our lab and how we used these findings for developing a novel antibiotic.