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APPLIED PRECISION WIDEFIELD DV CORE

LOCATION: Room 229

SYSTEM SPECIFICATIONS:

  • Imaging: very fast and sensitive inverted widefield microscope
  • Stand: Olympus IX71
  • XY-stage: motorized
  • Fully equipped for live imaging, temperature and CO2 controlled
  • Software: SoftWorx 4.1.2

CAMERA SPECIFICATIONS:

  • Model: Photometrics CoolSNAP HQ2, interline transfer CCD
  • Effective number of pixels: 1392 x 1040
  • Cell size: 6.45µm x 6.45µm
  • Effective Area: 8.98mm x 6.71mm
  • Max frame rate: 11 frames/second
  • Dynamic range: 14-bit
  • Quantum efficiency (peak): > 60%
  • Read out noise: 4-5 electrons

OBJECTIVES

Objective

Magnification / NA

Medium

Contrast

Working Distance

U Plan S Apo

10x / 0.40

air

-

3.1 mm

U Plan S Apo

20x / 0.75

air

-

0.6 mm

U Apo 340

40x / 1.35

oil

-

0.1 mm

Plan Apo N

60x / 1.42

oil

-

0.15 mm

UPLS Apo

100x / 1.40

oil

-

0.13 mm

Note: Extra 1.6 magnification lens available on the Olympus stand

SOLID STATE ILLUMINATION

Wavelengths

Availability

Laser type

Some dyes and use

390 / 18

YES

SSI

DAPI

438 / 24

YES

SSI

CyanFP

475 / 28

YES

SSI

GFP, Alexa488, CY2, FITC

513 / 17

YES

SSI

YellowFP

542 / 27

YES

SSI

TRITC

575 / 25

YES

SSI

mCherry, Alexa594

632 / 22

YES

SSI

DRAQ5, CY5, Alexa639

 

 

BANDPASS FILTERS (Emitters) + EYEPIECE FILTERS for Olympus stand

Fluorochromes

Availability

Emission

Mirror Unit Position

Filter Wheel

DAPI

YES

435 / 48

Manually select 1 or 3

“Standard” or “Alexa”

CFP

YES

475 / 24

Manually select 2

“Live”

GFP/FITC

YES

525 / 48

Manually select 1 or 3

“Standard”, “Alexa”, or “Live”

YFP

YES

548 / 22

Manually select 2

“Live”

TRITC

YES

597 / 45

Manually select 1

“Standard”

mCherry / Alexa594

YES

625 / 45

Manually select 2 or 3

“Live” or “Alexa”

Cy5

YES

679 / 34

Manually select 1 or 3

“Standard” or “Alexa”

Note: All bandpass filters are single-band.

MIRROR UNIT for Olympus stand (SELECT MANUALLY)

Fluorochromes

Availability

Position

DAPI / GFP / FITC / TRITC / Cy5

YES

1

CFP / YFP

YES

2

DAPI / GFP / FITC / mCherry / Alexa594 / Cy5

YES

3

DAPI / GFP / FITC / TRITC / mCherry / Alexa594 / Cy5

YES

6