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APPLIED PRECISION "OMX BLAZE"

LOCATION: Room 185

SYSTEM SPECIFICATIONS:

  • Imaging: Super-resolution (fixed and live), structured illumination (3D-SIM), Ring TIRF (works with PALM or TIRF techniques)
  • SIM resolution xy 100-160 nm; z 280-350 nm – Pixel resolution after reconstruction = 41 nm
  • Stand: DeltaVision OMX Optical Microscope (version 4)
  • XYZ-stage: motorized with 25 mm travel maximum. Piezo controlled Z axis system
  • UltimateFocus Hardware Autofocus module with FocusAssist
  • Fully equipped for live imaging, temperature and CO2 controlled
  • Software: DeltaVision OMX softWoRx

CAMERA SPECIFICATIONS:

  • 4 liquid cooled sCMOS cameras
  • Effective number of pixels: 512 x 512 for SIM imaging, 1024 x 1024 for widefield
  • Resolution: 0.08 μm pixel size
  • Total chip size: 5.5 Megapixels
  • Max frame rate: 400 frames/second
  • Rolling Shutter and Global Shutter modes
  • Dynamic range: 15 bit readout
  • Quantum efficiency (peak): ~ 65%

OBJECTIVES

Objective

Magnification / NA

Medium

Use for

Working Distance

U-PLAN S-APO

100x / 1.40

oil

Widefield + DIC

0.12 mm

Plan Apo N

60x / 1.42

oil

3D-SIM

0.15 mm

APON

60x / 1.49

oil

TIRF

0.1 mm

UPLS Apo

60x / 1.20

water

(to be delivered)

0.28 mm

LASERLINES (SOLID STATE ILLUMINATION)

FIX wavelengths (nm)

Availability

Laser type

Some dyes and use

405

YES

InsightSSI

DAPI

445

YES

InsightSSI

CyanFP

488

YES

InsightSSI

GFP, Alexa488, CY2

514

YES

InsightSSI

YellowFP

568

YES

InsightSSI

RedFPs, Alexa568

642

YES

InsightSSI

DRAQ5, CY5, Alexa647

EMISSION FILTERS

Fluorochromes

Availability

Bandpass

Emission

Drawer

DAPI

YES

Single-band

435 / 31

BGR

CFP

YES

Single-band

477 / 35

CYR

GFP / FITC

YES

Single-band

528 / 48

BGR

YFP

YES

Single-band

541 / 22

CYR

mCherry / Alexa568

YES

Single-band

609 / 37

BGR / CYR

Cy5 / Alexa647

YES

Single-band

683 / 40

BGR