Navigation mit Access Keys

Main Content

Main Content

LEICA POINT SCANNING CONFOCAL “SP5-II-MATRIX“

LOCATION: Room 187

SPECIFICATIONS:

  • Imaging: Confocal for live imaging; FRAP
  • Stand: Leica DMI 6000B with adaptive focus
  • XY-stage: motorized
  • Equipped with 2 HyD
  • Fully equipped for live imaging, temperature and CO2 controlled
  • Conventional scanner and resonant (ultra-fast, for reduced photodamage) scanner
  • AOBS serves as a tunable dichroic for all lasers
  • Software: LAS AF version 2.6.0.7266

 

 

OBJECTIVES

Objective

Magnification / NA

Medium

Availability

AFC

Working Distance

HC PL Fluotar

10x / 0.30

air

Yes

Yes

11 mm

HCX IRAPO L

25x / 0.95

water

On Demand

No

2.40 mm

HCX PLAN APO CS

40x / 1.10

water

Yes

No

0.63 mm

HCX PLAN APO

63x / 1.20

water

Yes

No

0.22 mm

HCX PLAN APO lambda blue

63x / 1.40-0.60

oil

Yes

Yes

0.10 mm

HCX PLAN APO lambda blue

40x / 1.25

oil

Yes

Yes

0.10 mm

HCX PLAN APO CS

20x / 0.70

air

Yes

Yes

0.59 mm

 

 

LASERLINES

FIXED wavelengths

Availability

Laser type and power

Some dyes and use

405nm

YES

diode laser, 50 mW

DAPI

458nm

YES

Argon laser, 5 mW

CyanFP

476nm

YES

Argon laser, 5 mW

 

488nm

YES

Argon laser, 20 mW

GFP, Alexa488, CY2, OregonGreen

496nm

YES

Argon laser, 5 mW

 

514nm

YES

Argon laser, 20 mW

YellowFP

561nm

YES

DPSS laser, 20 mW

RedFPs, Alexa568

633nm

YES

HeNe laser, 10 mW

DRAQ5, CY5, Alexa648