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Nikon Ti2 "seqFISH"

LOCATION: Room 184

SPECIFICATIONS:

  • Imaging: very flexible and stable inverted widefield microscope
  • Stand: Ti2-E Inverted with perfect focus and large field of view (F-mount)
  • XY-stage: fully motorized
  • Fully equipped for live imaging, temperature and CO2 controlled
  • Software: NIS Elements with JOBS
  • Camera: Photometrics Prime 95B with large field of view of 25 mm, F-mount, 11um pixel size
  • Light source: Lumencor SpectraX; Lasers for sequential FISH

OBJECTIVES

Objective

Magnification / NA

Medium

Contrast

Working Distance

CFI Plan Apo Lamda

10x / 0.45

air

standard DIC

4 mm

CFI Plan Apo Lamda

60x / 1.4

oil

HighRes DIC

0.13 mm

CFI Plan Apo Lamda

100x / 1.45

oil

HighRes DIC

0.13 mm

Note: Extra 1.5 magnification lens available on the Nikon Ti2

 

ILLUMINATION

   Wavelengths

Availability

   Type

Some dyes and use

405

on demand

Solid State Laser

DAPI

473

on demand

Solid State Laser

GFP, Alexa488, CY2, FITC

555

on demand

Solid State Laser, 1W

TRITC, strong for bleaching

638

on demand

Solid State Laser

DRAQ5, CY5, Alexa647

750

on demand

Solid State Laser

Cy7, Alexa750

395/25

YES

Solid State Light

DAPI

440/20

YES

Solid State Light

CFP

470/24

YES

Solid State Light

GFP, Alexa488, CY2, FITC

510/25

YES

Solid State Light

YFP

550/15

YES

Solid State Light

TRITC, Cy3

575/25

YES

Solid State Light

on demand (mCherry)

640/30

YES

Solid State Light

DRAQ5, CY5, Alexa639

 

 

DICHROIC MIRROR

5x Penta-bandpass: LED-DA/FI/TR/Cy5/Cy7-5X-A (DAPI / FITC / TRITC / Cy5 / Cy7 - Pinkel Penta)

Fluorochromes

Availability

Mirror (nm)

Transmission (nm)

DAPI

YES

414-450

420-460

GFP, Alexa488

YES

500-530

510-531

RFP, Alexa568

YES

580-611

590-624

Cy5, Alexa647

YES

661-701

677-712

Cy7, Alexa750

YES

769-850

769-850

 

 

EMISSION FILTERS in front of the camera

Fluorochromes

Availability

Bandpass

Transmission (nm)

DAPI

YES

single BP

435-485

GFP, Alexa488

YES

single BP

515-555

RFP, Alexa568

YES

single BP

565-615

Cy5, Alexa647

YES

single BP

peak 670