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TissueVision "TissueCyte"

LOCATION: Room 182

SYSTEM SPECIFICATIONS:

  • Imaging: Serial two-photon tomography
    • Whole organ imaging at high resolution without clearing procedures
    • No working distance limitations due to included microtome
  • Visualization of tissue with & without labeling by 2 photon microscopy
    • Auto fluorescence enables visualization of sample tissue and structures within the tissue due to differences in auto fluorescence emission spectra
    • Excitation of different fluorophores with one 2photon excitation wavelength and highly efficient emission detection with up to 3 non-descanned detectors.
  • Detection with 1664 x 1664 or 832 x 832 pixel/ frame
  • Typical x/y resolution: 1 to 2 microns per pixel. Typical z resolution:10 to 50 microns

    The software pipeline for:

    - Image reconstruction in MATLAB using StitchIt

    (https://github.com/BaselLaserMouse/StitchIt)

    - Visualisation of full resolution data sets using Arivis Vision-4D

    (https://www.arivis.com/en/imaging-science/arivis-vision4d)

    or the open-source tool, MASIV (https://github.com/alexanderbrown/masiv)

    is now available.

     

 

OBJECTIVES

Objective

Magnification / NA

Dipping medium

Use for

Working Distance

Zeiss Plan Apo

20x / 1.0

Water

Vis-IR

2.4mm

Zeiss Plan Apo

16x / 0.8

Water

Vis-IR

3mm

 

 

 

TUNABLE ULTRAFAST LASERS

Type/Model

Peak Power

Pulse Width

useful tuning Range

Average Power

Repetition rate

Mai Tai eHP

> 450 kW

< 70 fs

750 -960nm

> 2.5 W

80 MHz

 

 


EMISSION FILTERS

Fluorochromes

 

670 nm multiphoton short-pass dichroic beam splitter

Dichroic

default detector filter

DAPI, CFP

          SP 500

---

GFP / FITC / YFP

LP 500

---

mCherry / Cy5 / A647

LP 560

---

 

 detection filters can be customized.