Navigation mit Access Keys

Main Content

Main Content

ZEISS POINT SCANNING CONFOCAL “LSM800 Inverted“

LOCATION: Room 725a

SPECIFICATIONS:

  • Imaging: Confocal for fixed and large samples
  • Stand: Zeiss Axio Observer
  • 2 GaAsP PMT detectors, no transmitted light PMT
  • XY-stage: motorized
  • Integrated stitching tool
  • Software: Zen Blue 2.6

OBJECTIVES

Objective

Magnification / NA

Medium

Contrast

Working Distance

PLAN APO

5x / 0.16

air

-

12.1 mm

PLAN APO

10x / 0.45

air

-

2 mm

PLAN APO

25x / 0.80

imm

-

0.57 mm

PLAN APO

40x / 1.3

oil

-

0.21 mm

PLAN APO

63x / 1.40

oil

-

0.19 mm

LASERLINES

FIXED wavelengths

Availability

Laser type

Some dyes and use

405nm

YES

solid state, 5mW

DAPI

488nm

YES

solid state, 10mW

GFP, Alexa488, CY2

561nm

YES

solid state, 10mW

RedFPs, Alexa568

639nm

YES

solid state, 5mW

DRAQ5, CY5, Alexa648

FILTERCUBES

Fluorochromes

Availability

Excitation

Dichroic

Emission

Filter No

Blue

YES

G 365

FT 395

BP 445/50

49 DAPI

Green

YES

BP 470/40

FT 495

BP 525/50

38HE eGFP

Red

YES

BP 546/12

FT 560

BP 575-640

20 Rhodamine

 

Extra Emission filters: BP 505-530 and BP 575-615 IR