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ZEISS lightsheet microscope “Z1 lightsheet“

LOCATION: Room 183

SPECIFICATIONS:

    • Imaging: Lightsheet for live & fixed large samples;
    • Stand: Zeiss Z1 lightsheet microscope
    • XYZ & rotation-stage: motorized
    • Fully equipped for live-imaging, Temperature and CO2 controlled
    • Integrated multi-view reconstruction, deconvolution
    • Software: Zen 2010 for light sheet microscopy

    CAMERA SPECIFICATIONS:

    • 2 liquid cooled sCMOS cameras
    • Effective number of pixels: 1920 x 1920
    • Resolution: 0.23 μm pixel size
    • Max frame rate: 30 frames/second
    • Dynamic range: 16 bit readout
    • dynamic range of 36 000 : 1
    • extreme low readout noise of 0.8 e- med
    • quantum efficiency above 70%

    OBJECTIVES

    Objective

    Magnification / NA

    Medium

    Chamber/RI

    Working Distance

    PLAN APO

    5x / 0.16

    air

    water 1.33

    5.1 mm

    PLAN APO

    20x / 1.0

    water dipping

    water 1.33

    2.4 mm

    PLAN APO

    40x / 1.0

    water dipping

    water 1.33

    2.5 mm

    PLAN APO

    63x / 1.40

    water dipping

    water 1.33

    2.1 mm

    PLAN APO

    20x / 1.0

    clarity dipping

    clarity 1.46

    5.6 mm

    LASERLINES

    FIXED wavelengths

    Laser type

    Some dyes and use

    405nm

    solid state, 5mW

    DAPI

    488nm

    solid state, 10mW

    GFP, Alexa488, CY2

    561nm

    solid state, 10mW

    RedFPs, Alexa568, Cy3

    638nm

    solid state, 5mW

    DRAQ5, CY5, Alexa648

     Fluorescese filter sets

    Blue/green

    405 & 488

    LP 510

    BP 420-470

    BP 525-545

    Green/red1

    488 & 561

    LP 560

    BP 505-545

    BP 575-615

    Green/red2

    488 & 561

    LP 560

    BP 505-545

    LP 585

    Green/far red

    488 & 638

    LP 560

    BP 505-545

    LP 660