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I6: Introduction to Biophysical Methods

(2 days; 1 CP; Spring 2021)

This course is exceptionally offered as a 2 day online course in the Spring Semester 2021. The credit points can be obtained via Learning Contract.

Registration via phd-biozentrum(at)unibasto make life hard for spam

Ludovit Zweifel, Timothy Sharpe

This online course offers an introduction to the biophysical methods available for biological research in the Biozentrum. It will comprise two days of lectures and online demonstrations to cover theory, practice, application and data analysis for each technique. The aim of the course is to help students to identify those techniques that will make an impact in their own projects, and to give them sufficient understanding to be able to discuss and plan experiments.

The course will cover the following topics and techniques:

  • Stability screening for proteins (DSF, DLS, CD)
  • Measurement of biomolecular interactions (ITC, SPR, MST, Fluorescence, FCCS)
  • Characterising mass and size distributions (AUC, MALS, Mass Photometry, FCS, DLS)
  • In Vivo and Single Molecule Biophysics (FLIM, SM-FRET, FCS, FCCS)