Navigation mit Access Keys

Main Content

Olympus SpinSR (CSU-W1)

LOCATION: Room 187

SYSTEM SPECIFICATIONS:

  • Imaging: Fast imaging with low phototoxicity;
  • Stand: Olympus IX83
  • XY-stage: motorized
  • Not yet equipped for live imaging (no temperature and CO2 incubation)
  • Software: cellSens

CAMERA SPECIFICATIONS:

  • Model: Hamamatsu Flash4 V3, sCMOS
  • Effective number of pixels: 2048 x 2048
  • Cell size: 6.5µm x 6.5µm
  • Effective Area: 13.312mm x 13.312mm
  • Max frame rate: 100 frames/second
  • Dynamic range: 16 bit
  • Quantum efficiency (peak): > 82% (@560nm)
  • Read out noise: 0.8 - 1.6 electrons (depending on fps)

OBJECTIVES

Objective

Magnification / NA

Medium

Contrast

Working Distance

UPL X APO
4x

4x / 0.16

air

-

13 mm

UPL S APO
30x

30x / 1.05

silicone

DIC

0.8 mm

UPL APO
60 XOHR

60x / 1.5

oil

-

0.11 mm

UPL APO
100 XOHR

100x / 1.5

oil

-

0.12 mm

LASERLINES

FIXED wavelengths

Availability

Laser type

Some dyes and use

405nm

YES

diode laser

DAPI

488nm

YES

diode laser

GFP, Alexa488, CY2

561nm

YES

diode laser

RedFPs, Alexa568

640nm

YES

diode laser

DRAQ5, CY5, Alexa648

FILTERCUBES

Fluorochromes

Availability

Excitation

Dichroic

Emission

Filter No

DAPI

YES

-

-

-

-

GFP

YES

-

-

-

-

RFP

YES

-

-

-

-

Cy5

YES

-

-

-

-

BANDPASS FILTERS (Emitters)

Fluorochromes

Availability

Bandpass

Emission

Position No

 

 

Main Content