Olympus SpinD (CSU-W1) with Photomanipulation/FRAP
LOCATION: Room 07.079
SYSTEM SPECIFICATIONS:
- Imaging: Fast imaging with low phototoxicity;
- Stand: Olympus IX83
- XY-stage: motorized
- Software: cellSens
- Yokogawa CSU-W1 confocal scan head (50um disk)
CAMERA SPECIFICATIONS:
- Model: Hamamatsu ORCA-Fusion, sCMOS
- Effective number of pixels: 2304 x 2304
- Cell size: 6.5µm x 6.5µm
- Effective Area: 14.976mm x 14.976mm
- Max frame rate: 100 frames/second
- Dynamic range: 16 bit
- Quantum efficiency (peak): > 80% (@550nm)
- Read out noise: 0.7 - 1.4 electrons (depending on fps)
OBJECTIVES | ||||
Objective | Magnification / NA | Medium | Contrast | Working Distance |
UPL X APO | 4x / 0.16 | air | - | 13 mm |
UPL X APO | 10x / 0.4 | air | - | 3.1 mm |
UPL S APO | 30x / 1.05 | silicone | - | 0.8 mm |
UPL APO | 60x / 1.5 | oil | - | 0.11 mm |
UPL APO | 100x / 1.5 | oil | - | 0.12 mm |
LASER LINES | |||
FIXED wavelengths | Availability | Laser type | Some dyes and use |
405nm | YES | diode laser | - |
488nm | YES | diode laser | - |
561nm | YES | diode laser | - |
640nm | YES | diode laser | - |
EMISSION FILTERS in front of camera 1 | |||
Fluorochromes | Availability | Bandpass | Transmission (nm) |
DAPI | YES | single BP | 422-472 |
GFP, Alexa488 | YES | single BP | 500-550 |
RFP, Alexa568 | YES | single BP | 580-653 |
Cy5, Alexa647 | YES | single BP | 665-705 |
Beam splitter when using dual camera mode: Long pass 561nm
EMISSION FILTERS in front of camera 2 | |||
Fluorochromes | Availability | Bandpass | Transmission (nm) |
DAPI | YES | single BP | 422-472 |
GFP, Alexa488 | YES | single BP | 500-550 |