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Leica LMD7 Laser Micro-Dissection Microscope

LOCATION: Room 07.090

SPECIFICATIONS:

  • Leica LMD7 is a laser microdissection system enabling users to isolate specific single cells or entire areas of tissue. 
    • contact-free and contamination-free dissection
    • specimen collection by gravity
  • Diode-pumped solid state laser (349nm) for microdissection
  • LMD Scanning stage Ultra LMT350 including collection devices fitting:
    • 4 slides specimen holder
    • multi-collector for up to four 0.2 mL or 0.5 mL standard PCR tubes with flat cap
    • one 1.5 mL PCR tube with filled cap for direct contact pick-up
    • one 8-strip tube cap
    • one 12-strip tube cap
    • non-skirted or semi-skirted 96 well PCR plates
  • Upper stage (Specimen level, LMT1):
    • Travel range: 120 mm (x1) x 80 mm (y1)
    • Repeatability: <= 0.5 microns
    • Accuracy: <= 2 microns
  • Lower stage (Collector level, LMT2):
    • Travel range: 100 mm (x2) x 112 mm (y2)
    • Repeatability: <= 5 microns
    • Accuracy: <= 10 microns
    • Velocity range: 100 nm/s - 200 mm/s
    • Acceleration range:  100m/s2 - 1m/s2
  • Equipped with CoolLED pE 300 white illumination system for fluorescence microscopy
  • K3M monochrome CMOS camera (12 bits)
  • LAS X software, LMD Database, LIF-Browser and software dongle USB
OBJECTIVES
ObjectiveMagnification / NAMediumContrastAdaptive Focus Control (AFC)Working DistanceNotes
HC PL FL 1.25x / 0.04AirBF, Ph, Fluo-3.7 mmWith and without coverglass
HC PL FL 10x / 0.30AirBF, DIC, Ph, Fluoyes11.0With and without coverglass

HC PL FL L CORR

(with correction ring)

20x / 0.40Air

BF, DIC, Ph, DF, IMC, Fluo

IMC: Integrated Leica Modulation Contrast

yes7.5 - 6.2 mmWith and without coverglass

HC PL FLUOTAR L CORR XT

(with correction ring)

40x / 0.60Air

BF, DIC, Ph, DF, IMC, Fluo

IMC: Integrated Leica Modulation Contrast

-3.3 - 1.9 mmWith and without coverglass

HC PL FLUOTAR L CORR XT

(with correction ring)

63x / 0.70Air

BF, DIC, Ph, DF, IMC, Fluo

IMC: Integrated Leica Modulation Contrast

-2.6-1.8 mmWith coverglass

Filtercube:

FluorochromesAvailabilityFilter No
LMD-DAPIYES11505261
GFP for LMDYES11532651
LMD-Alexa594YES11505262