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Data Analysis FlowJo dongle is accessible after online booking on PPMS . One license is installed in FACS room (10.040). Additional FlowJo portal licenses are available for purchase via DBM Flow Team . Other common software…

Instruments The instruments shown below are available in the Biophysics Facility (BF) of the Biozentrum and can be booked and used by all members of the University of Basel, following training by BF staff. The instruments…

Protein Filaments Applications To determine the structure of filaments and their subunits at high resolution. Method Grids holding purified filament samples are plunge-frozen in liquid ethane and imaged. The 3D structure(s)…

Cryo-FIB milling Application A specialized technique for TEM specimen preparation to mill a bulk specimen with focused gallium (Ga) ions. The target region of the specimen can be selectively thinned down to a desired shape…

Thin Sectioning Application To determine the ultrastructure of whole tissues and cells at ~2nm resolution by transmission electron microscopy (TEM). Method Tissues or cells are chemically fixed, dehydrated, embedded in resin…

Webcam We have uninstalled our webcam. You can get a nice view over Basel here: https://www.wetter.com/hd-live-webcams/schweiz/basel/54c645b2ed814/

Publications

Electron Tomography Application To obtain a 3D model for objects that are too thick or vary too much in size and shape for single particle electron microscopy (≥ 1 micrometer thick), e.g., small cells, cellular organelles…