LEICA POINT SCANNING CONFOCAL “SP8“ BioSafety Level 2
LOCATION: Room 09.171
SPECIFICATIONS:
- Imaging: Confocal for live imaging (L2)
- Stand: Leica DMI 6000B with adaptive focus
- XY-stage: motorized
- Equipped with 1 HyD and 2 PMTs
- Fully equipped for live imaging, temperature and CO2 controlled
- AOBS serves as a tunable dichroic for all lasers
- Software: LAS AF version 3
OBJECTIVES | |||||
Objective | Magnification / NA | Medium | Contrast | AFC | Working Distance |
HC PL Fluotar | 10x / 0.30 | air | DIC | Yes | 11 mm |
HC PL APO CS | 20x / 0.70 | air | DIC | Yes | 0.59 mm |
HC PL APO CS2 | 20x / 0.75 | imm | DIC | Yes | 0.67 mm |
HC PL APO CS | 40x / 1.10 | water | DIC | No | 0.65 mm |
HC PL APO CS2 | 63x / 1.40 | oil | DIC | Yes | 0.14 mm |
LASER LINES | |||
Wavelengths (nm) | Availability | Type | Some dyes and use |
405 | YES | Continuous wave laser, 50mW | Alexa405, DAPI, Hoechst |
488 | YES | Solid state laser, 20mW | Alexa488, GFP, Cy2, OregonGreen |
552 | YES | Solid state laser, 20mW | RedRFP, Alexa568 |
638 | YES | Solid state laser, 30mW | Alexa647, Cy5, DRAQ5 |
FILTER CUBES | ||||
Fluorochromes | Availability | Excitation | Emission | Filter No |
Blue (DAPI, Hoechst) | YES | Multi-band | 450 - 490 | A4 |
Green (GFP) | YES | Single-band | 500 - 550 | GFP |
Red (TexasRed, Alexa568, Alexa594) | YES | Single-band | 607-683 | TX2 |