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BD FACSAria IIIu Laser (Excitation) Detector LP Mirror BP Filter Fluorochrome Blue Laser 488nm A B C D E F 735 655 610 556 495 / 780/60 695/40 616/23 585/42 514/30 488/10 PE-Cy7 PerCP, PeCy5, PerCP-Cy5.5, 7-AAD PE-Texas Red,…

Stochastic promoter choice during differentiation of neurons Mammals, including humans, have relatively few genes (20-50’000) but millions of neurons with distinct cellular identities. The combinatorial expression of protein…

The Skin I Live in The forces that shape embryos, and how embryonic cells respond Embryos undergo dramatic morphogenetic movements during development. For example, C. elegans embryos begin as a compact ball of cells and…

FEI Talos F200C TEM Configuration 200kV FEG Optics. Cryo-capable with insertable/retactable cryo box. Gatan 626 side-entry cryo holders (LN2 temperature). FEI Ceta 16M Pixel CMOS camera. STEM function. The Talos instrument…

Applications Single particle analysis is generally used to determine the 3D structure of globular proteins and protein complexes at high resolution. Method Grids holding purified protein samples are plunge-frozen in liquid…

Where to find information on (image) data management General Research Data Management (RDM) information: Clickable overview to all different aspects of RDM: rdmkit.elixir-europe.org Recommended Metadata for Biological…

TFS Titan Krios G4 TEM Configuration The Titan Krios G4 from Thermo Fisher Scientific is a high-end transmission cryo-electron microscope designed for ultimate performance and productivity across a wide range of Life Science…

Video Engineered kinases as a tool for phosphorylation of selected targets in vivo "The Dawn of Life" Cells into organs. Forscherportrait Prof. Markus Affolter (German) University of Basel: Interview Prof. Dr. Markus…

Research projects We investigate the physiology of bacterial pathogens ( Salmonella , Pseudomonas , Staphylococcus ) in infected rodent and human tissues, focusing on single-cell behavior and potential applications for novel…

Movies Bacterial nanosized speargun Dynamics of T6SS sheath in Vibrio cholerae. (Basler et al., Nature 2012) Dueling between green Pseudomonas aeruginosa cells (ClpV-GFP) and killing of red Vibrio cholerae cells…