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Nikon Ti2 X-Light V3
LOCATION: Room 07.057
SYSTEM SPECIFICATIONS:
- Imaging: Fast imaging / tiling of full tissue sections not thicker than 50 um
- Confocal and widefield mode
- Confocal scanhead: X-light Crest v3
- Disk: 50 um pinhole size, 250 um spacing
- Stand: Nikon Ti2 with perfect focus system (PFS)
- XY-stage: motorized
- Software: NIS elements with JOBS
- Lightsource: Lumencor Celesta 7 channel including CFP/YFP and Cy7/A750
CAMERA SPECIFICATIONS:
- Model: Teledyne Photometrics Kinetix
- Mount: 25 mm, large field of view
- Effective number of pixels: 3200 x 3200 cropped to 2700 x 2700
- Pixel size: 6.5µm x 6.5µm
- Effective Area: 14.976mm x 14.976mm
- Max frame rate: 88 frames/second
- Dynamic range: 12 bit
- Read out noise: 1.2 electrons
OBJECTIVES | ||||
Objective | Magnification / NA | Medium | Contrast | Working Distance |
| CFI Plan Apochromat Lambda D | 4x / 0.2 | air | - | 20 mm |
CFP Plan Apo Lambda D | 10x / 0.45 | air | - | 4 mm |
CFI Plan Apo | 20x / 0.75 | air | - | 1 mm |
CFI Plan Apo Lambda S | 25x / 1.05 | silicon | - | 0.55 mm |
CFI Plan Apo Lambda | 60x / 1.4 | oil | - | 0.13 mm |
ILLUMINATION Laser excitation (Lumencore Celesta) | |
Wavelengths (+- 2 nm) | Some dyes and use |
405 | Alexa405, DAPI |
446 | CFP |
477 | Alexa488, GFP |
520 | YFP |
546 | Alexa555, 568, CY3 |
638 | Alexa647, CY5 |
742 | Alexa750, CY7 |
EXCITATION FILTERS in the X-Light v3 scanhead | |
Filter set (multi-band) | excitation (nm) |
| V3-DA/FI/TR/Cy5/Cy7 | 391/477/549/639/741 |
| V3-CFP/YFP | 449/520 |
| 5% transmission ND | for widefield mode only |
| DICHROIC MIRRORS in the X-Light v3 scanhead | |
| Filter set (multi-band) | dichroic mirror (nm) |
V3-DA/FI/TR/Cy5/Cy7 | 441/511/593/684/817 |
| V3-CFP/YFP | 471/539 |
EMISSION FILTERS in front of the camera | ||
Fluorochromes | Bandpass | Transmission (nm) |
DAPI | single BP | 438/24 |
| CFP | single BP | 485/20 |
GFP, Alexa488 | single BP | 511/20 |
| YFP | single BP | 560/25 |
CY3, Alexa555, 568 | single BP | 595/31 |
CY5, Alexa647 | single BP | 685/40 |
| CY7, Alexa750 | multiband | 421/511/593/684/817 |