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Zeiss Axio Scan.Z1 Slide Scanner
LOCATION: Room 07.090
SYSTEM SPECIFICATIONS:
- fully automated for high throughput
- Z-drive with 3mm usable focus range
- Magazine for 100 slides 26x76mm
- Precision scanning stage 300x100mm
- light source for bright field: Vis-LED
- light source for fluorescence: X-Cite Xyliss 720L (LED)
- for fixed samples
- Software: Zen 3.5
2 CAMERAS:
Model: 3CCD Hitachi HV-F202SCL for Axio Scan.Z1 (O)
Type: High-End 3CCD progressive scan color camera.
- Effective number of pixels: 1600 x 1200
- Cell size: 4.4 µm x 4.4 µm
- Effective Area: 7.04 mm x 5.28 mm
- Max frame rate: 30 frames/s RGB
- Dynamic range: 12 bit
- Sensitivity: 2000 Lux/F5.6
Model: Orca Flash 4.0 V2 for Axio Scan.Z1 (O)
Type: High-End monochrome with digital interface
- Effective number of pixels: 2048 x 2048
- Cell size: 6.5 µm x 6.5 µm
- Effective Area: 13.31 mm x 13.31 mm
- Spectral range: appr. 250 - 1000 nm
- Camera Link frame grabber
- full frame rate: 100 fps, at 20 ms,
- Dynamic range: 16 bit
- Quantum efficiency (at 600 nm): > 70%
- noise at min EM gain: 1.6 electrons rms
OBJECTIVES | ||||
Objective | Magnification / NA | Medium | Contrast | Working distance |
Fluar | 5x / 0.25 | air | - | 12.5 mm |
Plan-Apochromat | 10x / 0.45 | air | - | 2.1 mm |
Plan-Apochromat | 20x / 0.8 | air | - | 0.55 mm |
Plan-Apochromat | 40x / 0.95 | air | - | 0.25 mm |
FILTER SETS | |||||
Fluorochromes | Availability | Excitation | Dichroic | Emission | Filter set |
DAPI | YES | 376-398 | 409 | 417-477 | F36-513 |
eGFP | YES | 450-490 | 495 | 495-545 | 38 HE eGFP shift free (E) |
Cy3 | YES | 525-575 | 570 | 570-640 | 43 HE Cy3 shift free (E) |
Cy5 | YES | 625-655 | 660 | 665-715 | 50 Cy5 shift free (E) |
Cy7 | YES | 773-758 | 766 | 776-826 | F46-825 |