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ZEISS POINT SCANNING CONFOCAL “LSM800 Inverted“

LOCATION: Room 07.092

SPECIFICATIONS:

  • Imaging: Confocal for fixed and large samples
  • Stand: Zeiss Axio Observer
  • 2 GaAsP PMT detectors, no transmitted light PMT
  • XY-stage: motorized
  • Integrated stitching tool
  • Software: Zen Blue 2.6

OBJECTIVES

Objective

Magnification / NA

Medium

Contrast

Working Distance

PLAN APO

5x / 0.16

air

-

12.1 mm

PLAN APO

10x / 0.45

air

-

2 mm

PLAN APO

25x / 0.80

imm

-

0.57 mm

PLAN APO

40x / 1.3

oil

-

0.21 mm

PLAN APO

63x / 1.40

oil

-

0.19 mm

LASER LINES

Wavelengths (nm)

Availability

Type

Some dyes and use

405

YES

Solid state, 5mW

Alexa405, DAPI, Hoechst

488

YES

Solid state, 10mW

Alexa488, GFP, Cy2

555

YES

Solid state, 10mW

RedRFP, Alexa568

639

YES

Solid state, 5mW

Alexa647, Cy5, DRAQ5

FILTER CUBES

Fluorochromes

Availability

Excitation

Dichroic

Emission

Filter No

Blue

YES

G 365

FT 395

420 - 470

49 DAPI

Green

YES

450 - 490

FT 495

500 - 550

38HE eGFP

Red

YES

540 - 552

FT 560

575 - 640

20 Rhodamine