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ZEISS lightsheet microscope “Lightsheet 7“ LOCATION: Room 07.092 SPECIFICATIONS: Imaging: Lightsheet for live & fixed large samples Stand: Zeiss Z1 lightsheet microscope XYZ & rotation-stage: motorized Fully equipped for…

New users I have never used the FCF If you never booked instruments and services in one of the other core facilities you first need to create a user profile on our booking homepage. You can request it directly at the PPMS…

Near by Core Facility Platforms Resources to neigbouring core facilities housed at the Department of Biomedicine in Hebelstrasse and Mattenstrasse part of the University Hospital Basel Here you can find the link to the Flow…

BD FACSAria Fusion Laser (Excitation) Detector LP Mirror BP Filter Fluorochrome Blue Laser 488nm A B C 655 502 / 695/40 514/30 488/10 PerCP, PeCy5, PerCP-Cy5.5, 7-AAD, PI FITC, Alexa Fluor 488, GFP SSC Red Laser 633nm A B C…

BD FACSAria IIIu Laser (Excitation) Detector LP Mirror BP Filter Fluorochrome Blue Laser 488nm A B C D E F 735 655 610 556 495 / 780/60 695/40 616/23 585/42 514/30 488/10 PE-Cy7 PerCP, PeCy5, PerCP-Cy5.5, 7-AAD PE-Texas Red,…

BD LSR Fortessa Laser (Excitation) PMT LP Mirror BP Filter Fluorochromes Blue Laser 488nm A B C D 670 525 505 / 685/35 542/27 512/25 488/10 PerCP YFP GFP, FITC, Alexa Fluor 488 SSC Red Laser 640nm A B C 750 690 / 780/60…

BD FACSCanto II Laser (Excitation) Detector LP Mirror BP Filter Fluorochrome Blue Laser 488nm A B D E F 735 655 556 502 / 780/60 670LP 585/42 530/30 488/10 PE-Cy7 PerCP, PeCy5, PerCP-Cy5.5, 7AAD PE, Cy3 FITC, Alexa Fluor…

Instruments The instruments shown below are available in the Biophysics Facility (BF) of the Biozentrum and can be booked and used by all members of the University of Basel, following training by BF staff. The instruments…

Electron Tomography Application To obtain a 3D model for objects that are too thick or vary too much in size and shape for single particle electron microscopy (≥ 1 micrometer thick), e.g., small cells, cellular organelles…

Applications Single particle analysis is generally used to determine the 3D structure of globular proteins and protein complexes at high resolution. Method Grids holding purified protein samples are plunge-frozen in liquid…