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FEI Titan Krios TEM (300kV)

Top left: Front protective enclosure of the Titan Krios. Top right: Detail of the microscope from inside the enclosure, showing the complexity of the microscope mainframe. Bottom: Operation control system including microscope, camera and drift correction consoles.

Configuration
80-300kV, FEG. 12-grid liquid nitrogen-cooled stage, Gatan Quantum-LS Energy Filter (GIF) with a Gatan K2 Summit direct electron detector. The instrument is housed within an environmental enclosure to reduce thermal and acoustic influences and increase stability. Online drift-correction and pre-processing capabilities are installed on adjacent computers directly next to the console of the instrument. SerialEM is generally used for automated image acquisition.


Application
The Titan Krios is a high-end transmission electron microscope tailored for molecular and cellular imaging at cryo-temperatures. The high stability permits a full range of applications, including, single particle, helical filament analysis, and electron tomography at ultimate resolution. Cellular imaging of frozen-hydrated cell organelles and cells prepared under cryo-conditions allows observation of specimens while the integrity in their native state is maintained. Applying an energy filter allows zero loss imaging to exclude inelastically scattered electrons. This can improve both contrast and resolution of an image.

Titan Krios Specifications 

Accelerating Voltage

300 kV

Electron Source

Schottky S-FEG

Stage Tilt Range

± 70°

C1 Apertures

2000, 70, 50 and 30 µm

C2 Apertures

150, 100, 70 and 50 µm

Objective Apertures

100, 70 µm

Operating Temperature

~80 K

Information Limit at 300kV at 0° / ±70° tilt

0.14 nm and 0.23 nm

Cs

2.7 mm

Number of cartridge positions for grids

12 total

Sample exchange time

~3 mins from autoloader cassette to column

Imaging filter and detector

GIF Quantum LS image filter fitted with a retractable K2 Direct Electron Detector;

·         Sensor size 3840 x 3712 pixels (14 megapixels)

·         Sensor active area 19.2 mm x 18.6 mm

·         Physical pixel size 5 µm

·         Sensor read out 400 full fps

·         Transfer speed to computer 40 full fps

·         DQE >0.5 at 0.5 physical Nyquist

·         Super-resolution with sub-pixel accuracy for 57 MP effective resolution

·         Counts individual image electrons (counting mode)

Acquisition software

SerialEM for single particle and tomography