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BD FACSAria IIIu

Laser (Excitation) Detector LP Mirror BP Filter Fluorochrome

Blue Laser 488nm

A

B

C

D

E

F

735

655

610

556

495

/

780/60

695/40

616/23

585/42

514/30

488/10

PE-Cy7

PerCP, PeCy5, PerCP-Cy5.5, 7-AAD

PE-Texas Red, PI

PE, Cy3

FITC, Alexa Fluor 488, GFP

SSC

Red Laser 633nm

A

B

C

735

685

/

780/60

710/50

660/20

APC-Cy7, APC-H7

Alexa Fluor 700

APC, Cy5, Alexa Fluor 647, DRAQ5

Violet Laser 405nm

A

B

C

550

502

/

593/40

530/30

450/40

BV605

Alexa Fluor 430, Pacific Orange, HorizonV500

Pacific Blue, DAPI, Hoechst, Alexa Fluor 405, HorizonV450

Yellow-Green Laser 561nm

A

B

C

D

735

630

600

565

780/60

670/14

610/20

586/25

PE-Cy7

mPlum,PE-Cy5, PE-Alexa Fluor 647

Cy3.5, dsRed, dTomato, Texas Red, mCherry

PE, RFP, Timer