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TFS Titan Krios G4 TEM
Configuration
The Titan Krios G4 from Thermo Fisher Scientific is a high-end transmission cryo-electron microscope designed for ultimate performance and productivity across a wide range of Life Science applications.
E-CFEG, (Cold FEG Gun) for low energy spread (<0.3 eV) 12 sample autoloader, aberration-free image shift (AFIS) and fringe-free imaging (FFI) Fitted with a dedicated Selectris X Energy Filter with a Falcon 4i direct electron detector and a Ceta 16M Camera.
EPU and Tomo5 and Tomo Live software are used for data acquisition and on the fly data assessment. Data management (via a DMP sever) is delivered using Athena, with Smart EPU and Quality Monitor software
Application
Top of the line cryo-TEM optimised for highest resolution data collection single particle and cryo-electron tomography. This enables the 3D visualisation of proteins and molecular complexes at the atomic level with the ability to further localize the molecular architecture of a cell in a close to native environment.
Titan Krios G4 Key Specifications
Electron source | E-CFEG |
TEM Info Limit (nm) | 0.12 nm at zero specimen tilt |
Cs | 2.7 mm |
Falcon 4i DQE | (0 Nyquist) = 0.92 at 300 kV |
Detectors | Falcon 4i fitted on a Selectris X TFS Imaging Filter. 4096x4096 pixels, 5.7cm2, 14 mm2 physical pixel. 320 fps internal frame rate. EER (electron event representation),MRC, Tiff formats.
Thermo Fisher Scientific Ceta 16M CCD 4096 x 4096, 14 µm pixel 16 bit dynamic range
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Titan Krios G4 overview, Word download