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TFS Titan Krios G4 TEM

The Titan Krios G4 from Thermo Fisher Scientific is a high-end transmission cryo-electron microscope designed for ultimate performance and productivity across a wide range of Life Science applications.
E-CFEG, (Cold FEG Gun) for low energy spread (<0.3 eV) 12 sample autoloader, aberration-free image shift (AFIS) and fringe-free imaging (FFI) Fitted with a dedicated Selectris X Energy Filter with a Falcon 4i direct electron detector and a Ceta 16M Camera.
EPU and Tomo5 and Tomo Live software are used for data acquisition and on the fly data assessment. Data management (via a DMP sever) is delivered using Athena, with Smart EPU and Quality Monitor software

Top of the line cryo-TEM optimised for highest resolution data collection single particle and cryo-electron tomography. This enables the 3D visualisation of proteins and molecular complexes at the atomic level with the ability to further localize the molecular architecture of a cell in a close to native environment.

Titan Krios G4 Key Specifications

Electron source


TEM Info Limit (nm)

0.12 nm at zero specimen tilt


2.7 mm

Falcon 4i DQE

(0 Nyquist) = 0.92 at 300 kV


Falcon 4i fitted on a Selectris X TFS Imaging Filter.

4096x4096 pixels, 5.7cm2, 14 mm2 physical pixel.

320 fps internal frame rate.

EER (electron event representation),MRC, Tiff formats.


Thermo Fisher Scientific Ceta 16M CCD

4096 x 4096, 14 µm pixel

16 bit dynamic range



Titan Krios G4 overview, Word download