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APPLIED PRECISION WIDEFIELD DV CORE
LOCATION: Room 07.051
SYSTEM SPECIFICATIONS:
- Imaging: very fast and sensitive inverted widefield microscope
- Stand: Olympus IX71
- XY-stage: motorized
- Fully equipped for live imaging, temperature and CO2 controlled
- Software: SoftWorx 4.1.2
CAMERA SPECIFICATIONS:
- Model: Photometrics CoolSNAP HQ2, interline transfer CCD
- Effective number of pixels: 1392 x 1040
- Cell size: 6.45µm x 6.45µm
- Effective Area: 8.98mm x 6.71mm
- Max frame rate: 11 frames/second
- Dynamic range: 14-bit
- Quantum efficiency (peak): > 60%
- Read out noise: 4-5 electrons
OBJECTIVES | ||||
Objective | Magnification / NA | Medium | Contrast | Working Distance |
U Plan S Apo | 10x / 0.40 | air | - | 3.1 mm |
U Plan S Apo | 20x / 0.75 | air | - | 0.6 mm |
U Apo 340 | 40x / 1.35 | oil | - | 0.1 mm |
Plan Apo N | 60x / 1.42 | oil | - | 0.15 mm |
UPLS Apo | 100x / 1.40 | oil | - | 0.13 mm |
Note: Extra 1.6 magnification lens available on the Olympus stand
ILLUMINATION | |||
Wavelengths (nm) | Availability | Type | Some dyes and use |
390/18 | YES | Solid state light | DAPI |
438/24 | YES | Solid state light | CyanFP |
475/28 | YES | Solid state light | GFP, Alexa488, Cy2, FITC |
513/17 | YES | Solid state light | YellowFP |
542/27 | YES | Solid state light | TRITC |
575/25 | YES | Solid state light | mCherry, Alexa594 |
632/22 | YES | Solid state light | DRAQ5, Cy5, Alexa639 |
BANDPASS FILTER (Emitters) + EYEPIECE FILTERS | |||||
Fluorochromes | Availability | Emission | Mirror unit position | Filter Wheel | |
DAPI | YES | 435/48 | Manually select 1 or 3 | "Standard" or "Alexa" | |
CFP | YES | 475/24 | Manually select 2 | "Live" | |
GFP/FITC | YES | 525/48 | Manually select 1 or 3 | "Standard", "Alexa" or "Live" | |
YFP | YES | 548/22 | Manually select 2 | "Live" | |
TRITC | YES | 597/45 | Manually select 1 | "Standard" | |
mCherry/Alexa594 | YES | 625/45 | Manually select 2 or 3 | "Live" or "Alexa" | |
Cy5 | YES | 679/34 | Manually select 1 or 3 | "Standard" or "Alexa" | |
Note: All bandpass filters are single-band.
MIRROR UNIT for Olympus stand (SELECT MANUALLY) | ||
Fluorochromes | Availability | Position |
DAPI / GFP / FITC / TRITC / Cy5 | YES | 1 |
CFP / YFP | YES | 2 |
DAPI / GFP / FITC / mCherry / Alexa594 / Cy5 | YES | 3 |
DAPI / GFP / FITC / TRITC / mCherry / Alexa 594 / Cy5 | YES | 6 |