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Nikon CSU-W1
LOCATION: Room 09.173 (BSL2)
SYSTEM SPECIFICATIONS:
- Imaging: Fast imaging with low phototoxicity
- Stand: Nikon Ti2 with perfect focus system (PFS)
- XY-stage: motorized
- Temperature and CO2 incubation
- Software: NIS elements with JOBS
- Yokogawa CSU-W1 confocal scan head (50um disk)
CAMERA SPECIFICATIONS:
- Model: 2x Hamamatsu ORCA-Fusion, sCMOS
- Effective number of pixels: 2304 x 2304
- Cell size: 6.5µm x 6.5µm
- Effective Area: 14.976mm x 14.976mm
- Max frame rate: 100 frames/second
- Dynamic range: 16 bit
- Quantum efficiency (peak): > 80% (@550nm)
- Read out noise: 0.7 - 1.4 electrons (depending on fps)
OBJECTIVES | ||||
Objective | Magnification / NA | Medium | Contrast | Working Distance |
CFI Plan Fluor | 4x / 0.13 | air | - | 17.2 mm |
CFP Plan Apo Lambda | 10x / 0.45 | air | - | 4 mm |
CFI Plan Apo DM Lambda | 20x / 0.75 | air | Ph2 | 1 mm |
CFI Plan Apo Lambda S | 25x / 1.05 | silicon | DIC | 0.55 mm |
CFI Apo LWD Lambda S | 40x / 1.15 | water | - | 0.61 - 0.59 mm |
CFI Plan Apo Lambda | 100x / 1.45 | oil | DIC | 0.13 mm |
ILLUMINATION | |||
Wavelengths (nm) | Availability | Type | Some dyes and use |
405 | YES | Diode laser | Alexa405, DAPI |
488 | YES | Diode laser | Alexa488, GFP |
561 | YES | Diode laser | Alexa568, RFP |
640 | YES | Diode laser | Alexa647, Cy5 |
395/25 | YES | Solid state light | DAPI |
440/20 | YES | Solid state light | CFP |
470/24 | YES | Solid state light | GFP, Alexa488, FITC |
508/25 | YES | Solid state light | YFP |
555/15 | YES | Solid state light | TRITC, Cy3 |
640/30 | YES | Solid state light | Alexa647, Cy5 |
DICHROIC MIRROR | |||
Fluorochromes | Availability | Mirror (nm) | Transmission (nm) |
DAPI | YES | 400-415 | 422-473 |
GFP, Alexa488 | YES | 483-497 | 503-545 |
RFP, Alexa568 | YES | 561-568 | 586-620 |
Cy5, Alexa647 | YES | 638-647 | 665-750 |
EMISSION FILTERS in front of the camera | |||
Fluorochromes | Availability | Bandpass | Transmission (nm) |
DAPI | YES | single BP | 417-477 |
GFP, Alexa488 | YES | single BP | 510-540 |
RFP, Alexa568 | YES | single BP | 574-626 |
Cy5, Alexa647 | YES | single BP | 671-745 |