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ZEISS POINT SCANNING CONFOCAL “LSM800 Inverted“
LOCATION: Room 07.092
SPECIFICATIONS:
- Imaging: Confocal for fixed and large samples
- Stand: Zeiss Axio Observer
- 2 GaAsP PMT detectors, no transmitted light PMT
- XY-stage: motorized
- Integrated stitching tool
- Software: Zen Blue 2.6
OBJECTIVES | ||||
Objective | Magnification / NA | Medium | Contrast | Working Distance |
PLAN APO | 5x / 0.16 | air | - | 12.1 mm |
PLAN APO | 10x / 0.45 | air | - | 2 mm |
PLAN APO | 25x / 0.80 | imm | - | 0.57 mm |
PLAN APO | 40x / 1.3 | oil | - | 0.21 mm |
PLAN APO | 63x / 1.40 | oil | - | 0.19 mm |
LASER LINES | |||
Wavelengths (nm) | Availability | Type | Some dyes and use |
405 | YES | Solid state, 5mW | Alexa405, DAPI, Hoechst |
488 | YES | Solid state, 10mW | Alexa488, GFP, Cy2 |
555 | YES | Solid state, 10mW | RedRFP, Alexa568 |
639 | YES | Solid state, 5mW | Alexa647, Cy5, DRAQ5 |
FILTER CUBES | |||||
Fluorochromes | Availability | Excitation | Dichroic | Emission | Filter No |
Blue | YES | G 365 | FT 395 | 420 - 470 | 49 DAPI |
Green | YES | 450 - 490 | FT 495 | 500 - 550 | 38HE eGFP |
Red | YES | 540 - 552 | FT 560 | 575 - 640 | 20 Rhodamine |