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ZEISS POINT SCANNING CONFOCAL “LSM880 Inverted“ WITH AIRYSCAN
LOCATION: Room 07.083
SPECIFICATIONS:
- Imaging: Confocal for live; large samples; Airyscan
- Stand: Zeiss Axio Observer
- XYZ-stage: motorized
- Fully equipped for live imaging, Temperature and CO2 controlled
- Integrated stitching tool
- Widefield camera to easily set tile experiments
- Airyscan FAST technique increases acquisition speed > 4x
- Airyscan SR technique increase by 1.7 your resolution.
- Software: Zen Blue
OBJECTIVES | ||||
Objective | Magnification / NA | Medium | Contrast | Working Distance |
PLAN APO | 10x / 0.45 | air | DIC | 2 mm |
PLAN APO | 25x / 0.80 | imm | DIC | 0.57 mm |
PLAN APO | 40x / 1.20 | sil/imm | DIC | 0.41 mm |
PLAN APO | 63x / 1.40 | oil | DIC | 0.19 mm |
LASER LINES | |||
Wavelengths (nm) | Availability | Type | Some dyes and use |
405 | YES | Solid state, 5mW | Alexa405, DAPI, Hoechst |
488 | YES | Solid state, 10mW | Alexa488, GFP, Cy2 |
555 | YES | Solid state, 10mW | RedRFP, Alexa568 |
639 | YES | Solid state, 5mW | Alexa647, Cy5, DRAQ5 |
FILTER CUBES | |||||
Fluorochromes | Availability | Excitation | Dichroic | Emission | Filter No |
Blue | YES | G 365 | FT 395 | 420 - 470 | 49 DAPI |
Green | YES | 450 - 490 | FT 495 | 500 - 550 | 38HE eGFP |
Red | YES | 540 - 552 | FT 560 | 575 - 640 | 20 Rhodamine |