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Nikon Ti2 "seqFISH"
LOCATION: Room 07.057
SPECIFICATIONS:
- Imaging: very flexible and stable inverted widefield microscope
- Stand: Ti2-E Inverted with perfect focus and large field of view (F-mount)
- XY-stage: fully motorized
- Fully equipped for live imaging, temperature and CO2 controlled
- Software: NIS Elements with JOBS
- Camera: Photometrics Prime 95B with large field of view of 25 mm, F-mount, 11um pixel size
- Light source: Lumencor SpectraX; Lasers for sequential FISH
OBJECTIVES | ||||
Objective | Magnification / NA | Medium | Contrast | Working Distance |
CFI Plan Apo Lamda | 10x / 0.45 | air | standard DIC | 4 mm |
CFI Plan Apo Lamda | 60x / 1.4 | oil | HighRes DIC | 0.13 mm |
CFI Plan Apo Lamda | 100x / 1.45 | oil | HighRes DIC | 0.13 mm |
Note: Extra 1.5 magnification lens available on the Nikon Ti2
ILLUMINATION | |||
Wavelengths (nm) | Availability | Type | Some dyes and use |
405 | YES | Solid state laser | DAPI |
488 | YES | Solid state laser | GFP, Alexa488, Cy2, FITC |
561 | YES | Solid state laser | TRITC, strong for bleaching |
640 | YES | Solid state laser | DRAQ5, Cy5, Alexa647 |
750 | YES | Solid state laser | Cy7, Alexa750 |
395/25 | YES | Solid state light | DAPI |
440/20 | YES | Solid state light | CFP |
470/24 | YES | Solid state light | GFP, Alexa488, Cy2 FITC |
510/25 | YES | Solid state light | YFP |
550/15 | YES | Solid state light | TRITC, Cy3 |
575/25 | YES | Solid state light | mCherry, on demand |
635/18 | YES | Solid state light | DRAQ5, Cy5, Alexa639 |
DICHROIC MIRROR 5x Penta-bandpass: LED-DA/FI/TR/Cy5/Cy7-5X-A (DAPI / FITC / TRITC / Cy5 / Cy7 - Pinkel Penta) | ||
Fluorochromes | Reflection to sample (nm) | Transmission to camera (nm) |
DAPI | 350-404 | 414-450 |
GFP, Alexa488 | 461-487.5 | 499.5-530 |
RFP, Alexa568 | 543-566 | 580-611 |
Cy5, Alexa647 | 626-644 | 661-701 |
Cy7, Alexa750 | 721-749 | 768.5-849.5 |
EMISSION FILTERS in front of the camera | |||
Fluorochromes | Availability | Bandpass | Transmission (nm) |
DAPI | YES | single BP | 432/36 |
GFP, Alexa488 | YES | single BP | 515/30 |
RFP, Alexa568 | YES | single BP | 595/31 |
Cy5, Alexa647 | YES | single BP | 680/42 |
| Cy7, Alexa750 | YES | single BP | 809/81 |
| penta-EM | YES | penta-band | FF01-432/515/595/681/809 |