LOCATION: Room 07.057

SPECIFICATIONS:

• Stand: Ti2-E Inverted with perfect focus and large field of view (F-mount)
• Fully motorized stage 
• Nano Z200 piezo from MCL
• Confocal scanhead: Yokogawa CSU-W1 with Uniformizer
  • disk: 50 um
• Light sources: 4 laser lines for confocal imaging, Lumencore SpectraX and BrixXHUB high-power laser combiner for Widefield imaging
• Software: NIS elements v 6.2 with JOBS
• Camera for widefield mode:
  • Photometrics Prime 95B (large field of view of 25 mm, F-mount, 11um pixel size)
    • intensity rolloff in the green channel using the 60x: at field number 25 (full chip): 25%, at field number 22: 15%
    • using the 10x at field number 22: 30%
• Camera for spinning disk mode: 
  • Hamamatsu ORCA Fusion (C-mount, connected by CoaXPress, 22mm FOV, high QE, 6.5um pixel size, fast readout, low noise)
• Incubator and temperature unit for live cell imaging: OKO-lab with OKO-TOUCH: S/N 748-3299 - factory date 25/06/2018
• Equipped with BiopTech FCS flow chamber, 3 Hamilton Serial MVPs, a Gilson pump for microfluidics experiments

OBJECTIVES

SPINNING DISK MODE:

Light sources:

4 Laser lines used for the spinning disk unit

Bleaching lasers: Laser Lines for excitation 

Main Dichroic Mirror in the confocal scan head

405/488/568/647 nm Yokogawa dichroic beamsplitter

Emission filter wheel in front of the Fusion camera

WIDEFIELD MODE

Light sources

LED excitation (Lumencore SpectraX) used for widefield imaging:

Main Dichroic Mirror

5x Penta-bandpass: LED-DA/FI/TR/Cy5/Cy7-5X-A (DAPI / FITC / TRITC / Cy5 / Cy7 - Pinkel Penta)

Emission filter wheel in front of the Photometrics Prime 95B camera