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ZEISS POINT SCANNING CONFOCAL “LSM700 Upright“
LOCATION: Room 07.083
SPECIFICATIONS:
- Imaging: Confocal for fixed samples
- Stand: Zeiss Axio Imager
- XYZ-stage: fully motorized
- Software: Zen black 2010
OBJECTIVES | ||||
Objective | Magnification / NA | Medium | Contrast | Working distance |
PLAN APO | 10x / 0.45 | air | DIC | 2 mm |
PLAN APO | 20x / 0.80 | air | DIC | 0.55 mm |
PLAN APO | 40x / 1.30 | oil | DIC | 0.21 mm |
PLAN APO | 63x / 1.40 | oil | DIC | 0.19 mm |
LASER LINES | |||
Wavelengths (nm) | Availability | Type | Some dyes and use |
405 | YES | Solid state, 5mW | Alexa405, DAPI, Hoechst |
488 | YES | Solid state, 10mW | Alexa488, GFP, Cy2 |
555 | YES | Solid state, 10mW | RedRFP, Alexa568 |
639 | YES | Solid state, 5mW | Alexa647, Cy5, DRAQ5 |
FILTER CUBES | |||||
Fluorochromes | Availability | Excitation | Dichroic | Emission | Filter No |
Blue | YES | G 365 | FT 395 | 420 - 470 | 49 DAPI |
Green | YES | 450 - 490 | FT 495 | 500 - 550 | 38HE eGFP |
Red | YES | 525 - 575 | FT 570 | 570 - 640 | 43HE Cy3 |
Extra Emission filters: BP 505-530 and BP 575-615 IR