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LEICA POINT SCANNING CONFOCAL “SP8“ BioSafety Level 2

LOCATION: Room 09.171

SPECIFICATIONS:

  • Imaging: Confocal for live imaging (L2)
  • Stand: Leica DMI 6000B with adaptive focus
  • XY-stage: motorized
  • Equipped with 1 HyD and 2 PMTs
  • Fully equipped for live imaging, temperature and CO2 controlled
  • AOBS serves as a tunable dichroic for all lasers
  • Software: LAS AF version 3

 

OBJECTIVES

Objective

Magnification / NA

Medium

Contrast

AFC

Working Distance

HC PL Fluotar

10x / 0.30

air

DIC

Yes

11 mm

HC PL APO CS

20x / 0.70

air

DIC

Yes

0.59 mm

HC PL APO CS2

20x / 0.75

imm

DIC

Yes

0.67 mm

HC PL APO CS

40x / 1.10

water

DIC

No

0.65 mm

HC PL APO CS2

63x / 1.40

oil

DIC

Yes

0.14 mm

LASER LINES

Wavelengths (nm)

Availability

Type

Some dyes and use

405

YES

Continuous wave laser, 50mW

Alexa405, DAPI, Hoechst

488

YES

Solid state laser, 20mW

Alexa488, GFP, Cy2, OregonGreen

552

YES

Solid state laser, 20mW

RedRFP, Alexa568

638

YES

Solid state laser, 30mW

Alexa647, Cy5, DRAQ5

FILTER CUBES

Fluorochromes

Availability

Excitation

Emission

Filter No

Blue (DAPI, Hoechst)

YES

Multi-band

450 - 490

A4

Green (GFP)

YES

Single-band

500 - 550

GFP

Red (TexasRed, Alexa568, Alexa594)

YES

Single-band

607-683

TX2