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Zeiss Axio Scan.Z1 Slide Scanner

LOCATION: Room 07.090

SYSTEM SPECIFICATIONS:

  • fully automated for high throughput
  • Z-drive with 3mm usable focus range
  • Magazine for 100 slides 26x76mm
  • Precision scanning stage 300x100mm
  • light source for bright field: Vis-LED
  • light source for fluorescence: X-Cite Xyliss 720L (LED)
  • for fixed samples
  • Software: Zen 3.5

2 CAMERAS:

Model: 3CCD Hitachi HV-F202SCL for Axio Scan.Z1 (O)

Type: High-End 3CCD progressive scan color camera.

  • Effective number of pixels: 1600 x 1200
  • Cell size: 4.4 µm x 4.4 µm
  • Effective Area: 7.04 mm x 5.28 mm
  • Max frame rate: 30 frames/s RGB
  • Dynamic range: 12 bit
  • Sensitivity: 2000 Lux/F5.6

Model: Orca Flash 4.0 V2 for Axio Scan.Z1 (O)

Type: High-End monochrome with digital interface

  • Effective number of pixels: 2048 x 2048
  • Cell size: 6.5 µm x 6.5 µm
  • Effective Area: 13.31 mm x 13.31 mm
  • Spectral range: appr. 250 - 1000 nm
  • Camera Link frame grabber
  • full frame rate: 100 fps, at 20 ms,
  • Dynamic range: 16 bit
  • Quantum efficiency (at 600 nm): > 70%
  • noise at min EM gain: 1.6 electrons rms

OBJECTIVES

Objective

Magnification / NA

Medium

Contrast

Working distance

Fluar

5x / 0.25

air

-

12.5 mm

Plan-Apochromat

10x / 0.45

air

-

2.1 mm

Plan-Apochromat

20x / 0.8

air

-

0.55 mm

Plan-Apochromat

40x / 0.95

air

-

0.25 mm

FILTER SETS

Fluorochromes

Availability

Excitation

Dichroic

Emission

Filter set

DAPI

YES

376-398

409

417-477

F36-513

eGFP

YES

450-490

495

495-545

38 HE eGFP shift free (E)

Cy3

YES

525-575

570

570-640

43 HE Cy3 shift free (E)

Cy5

YES

625-655

660

665-715

50 Cy5 shift free (E)

Cy7 YES 773-758 766 776-826 F46-825