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Leica Viventis SCAPE
LOCATION: Room 07.081
SPECIFICATIONS:
- Single objective lightsheet based in OPM/SCAPE technology
- DMi8 stand with LMT200-T high-precision scanning stage
- K3M camera for widefield
- sCMOS Kinetix 22 cameras for SCAPE mode (2 cameras)
- Pixel size: 6.5 µm
- Max. pixel format: 2048 x 2048
- QE up to 95%
- OKOlab incubation for environment controls
- Illumination:
- pE400Max LEDs for widefield (400, 450, 545, 635 nm)
- 4 laser lines for SCAPE (405, 488, 561 and 638 nm)
- Objectives 1.6x and 10x for widefield
- 40x objective for widefield and SCAPE modes, equipped with motorized correction collar and automatic water dispenser
- Light sheet thickness: 1.5 µm or 3.4 µm
- Different c-mounts available: -1x, 0.5x and 0.35x
| OBJECTIVES | |||
| Objective | Magnification / NA | Medium | Working Distance |
| 1.6x | 1.6x / 0.05 | air | 3.4 mm |
| 10x | 10x / 0.32 | air | 11.2 mm |
| Plan Apo 40x | 40x / 1.1 | water | 620 µm |
| LASER LINES | ||
| Fixed wavelengths (nm) | Laser type | Example dyes |
| 405 | 50 mW | DAPI, Hoechst |
| 488 | 150 mW | GFP, Alexa 488 |
| 561 | 120 mW | Alexa 568, Cy3 |
| 638 | 150 mW | Alexa 647, Cy5 |
| EMISSION FILTERS (SCAPE) | |
| Image splitter cubes | Wavelength |
| Reflected Camera | 442/35, 525/40 |
| Transmitted Camera | 590/40, 702/68 |
| Fast filter wheel (for -1x c-mount) | Wavelength |
| Reflected Camera | 460/80, 535/70 |
| Transmitted Camera | 590/50, 700/75 |