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ZEISS POINT SCANNING CONFOCAL “LSM700 Upright“

LOCATION: Room 07.083

SPECIFICATIONS:

  • Imaging: Confocal for fixed samples
  • Stand: Zeiss Axio Imager
  • XYZ-stage: fully motorized
  • Software: Zen black 2010

OBJECTIVES

Objective

Magnification / NA

Medium

Contrast

Working distance

PLAN APO

10x / 0.45

air

DIC

2 mm

PLAN APO

20x / 0.80

air

DIC

0.55 mm

PLAN APO

40x / 1.30

oil

DIC

0.21 mm

PLAN APO

63x / 1.40

water

DIC

0.19 mm

LASER LINES

Wavelengths (nm)

Availability

Type

Some dyes and use

405

YES

Solid state, 5mW

Alexa405, DAPI, Hoechst

488

YES

Solid state, 10mW

Alexa488, GFP, Cy2

555

YES

Solid state, 10mW

RedRFP, Alexa568

639

YES

Solid state, 5mW

Alexa647, Cy5, DRAQ5

FILTER CUBES

Fluorochromes

Availability

Excitation

Dichroic

Emission

Filter No

Blue

YES

G 365

FT 395

420 - 470

49 DAPI

Green

YES

450 - 490

FT 495

500 - 550

38HE eGFP

Red

YES

525 - 575

FT 570

570 - 640

43HE Cy3

 

Extra Emission filters: BP 505-530 and BP 575-615 IR