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Leica Viventis SCAPE

LOCATION: Room 07.081

SPECIFICATIONS:

  • Single objective lightsheet based in OPM/SCAPE technology
  • DMi8 stand with LMT200-T high-precision scanning stage
  • K3M camera for widefield
  • sCMOS Kinetix 22 cameras for SCAPE mode (2 cameras)
    • Pixel size: 6.5 µm
    • Max. pixel format: 2048 x 2048
    • QE up to 95%
  • OKOlab incubation for environment controls
  • Illumination:
    • pE400Max LEDs for widefield (400, 450, 545, 635 nm)
    • 4 laser lines for SCAPE (405, 488, 561 and 638 nm)
  • Objectives 1.6x and 10x for widefield
  • 40x objective for widefield and SCAPE modes, equipped with motorized correction collar and automatic water dispenser 
  • Light sheet thickness: 1.5 µm or 3.4 µm
  • Different c-mounts available: -1x, 0.5x and 0.35x
OBJECTIVES   
ObjectiveMagnification / NAMediumWorking Distance
1.6x1.6x / 0.05air3.4 mm
10x10x / 0.32air11.2 mm
Plan Apo 40x40x / 1.1water620 µm
LASER LINES
Fixed wavelengths (nm)Laser typeExample dyes
40550 mWDAPI, Hoechst
488150 mWGFP, Alexa 488
561120 mWAlexa 568, Cy3
638150 mWAlexa 647, Cy5