Protein Filaments Applications To determine the structure of filaments and their subunits at high resolution. Method Grids holding purified filament samples are plunge-frozen in liquid ethane and imaged. The 3D structure(s)…
Cryo-FIB milling Application A specialized technique for TEM specimen preparation to mill a bulk specimen with focused gallium (Ga) ions. The target region of the specimen can be selectively thinned down to a desired shape…
Thin Sectioning Application To determine the ultrastructure of whole tissues and cells at ~2nm resolution by transmission electron microscopy (TEM). Method Tissues or cells are chemically fixed, dehydrated, embedded in resin…
FEI Titan Krios TEM (300kV) Top left: Front protective enclosure of the Titan Krios. Top right: Detail of the microscope from inside the enclosure, showing the complexity of the microscope mainframe. Bottom: Operation…
Thermo Fisher Scientific Glacios TEM Configuration 200kV X-FEG optics. C-TWIN objective lens. Autoloader for multi-grid loading at cryo-LN2 temperature and automated exchange of grid to column. The Glacios instrument is…
Thermo Fisher Scientific Talos L120C Configuration The Thermo Fisher Scientific™ Talos L120C G2 is a 120 kV thermionic transmission electron microscope designed for performance and productivity across a wide range of…
FEI Tecnai G2 Spirit TEM Configuration BioTWIN lens configuration for increased contrast. 120kV TEM microscope (operated at 80kV) with a LaB6 filament and an EMSIS Veleta camera (top, side-mounted). The Camera operates using…
Leica STELLARIS 8 Cryo-Confocal Microscope Configuration STELLARIS 8 is a true confocal point scanning system, fitted with a cryo-stage. It includes a White Light Laser (WLL) with a pulse picker as excitation light source,…
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