Navigation mit Access Keys

I5: Introduction to Protein Interaction Analysis by Affinity Purification – Mass Spectrometry – 34604

(3 days; 1 CP; Spring 2023)

Thomas Bock, Alexander Schmidt

3-day practical course on affinity purification - mass spectrometry (AP-MS) based investigation of protein-protein interactions and posttranslational modifications. This course focuses on the protein interaction analysis of protein phosphatase complexes, which have been linked to the regulation of a number of fundamental biological processes (e.g. cell growth, apoptosis and more) and are involved in cellular transformation during tumorgenesis.

Day 1: Sample preparation
Seminar: Introduction to AP-MS workflows, advantages/disadvantages of different protein affinity tags/enrichment strategies

Hands-on training: Affinity purification of one target protein per student (each student canbring one additional protein of interest for purification via affinity resins), protein digestion over night

Day 2: Mass spectrometric analysis
Seminar: Introduction to mass spectrometry (MS) and its applications in molecular biology

Hands-on training: Solid-phase extraction of peptides, mass spectrometric measurement

Day 3: Data analysis
Seminar: Introduction to MS-data analysis, principles of protein identification