Fluorescence microscopy, an important and widely used tool in biological research, has witnessed a true renaissance since the invention of methods that circumvent the so-called diffraction limit, namely super-resolution techniques. In resonance with these advancements, my work combines advanced high-resolution microscopy with novel approaches from the field of DNA Nanotechnology to push the limits of light microscopy and apply it to cell-biological research. In this presentation, I will describe current advances in DNA-based super-resolution microscopy, including novel immuno-labeling strategies, proximity detection, spatial imaging range (from close to the coverslip to whole cells and potentially tissues), image acquisition speed, and spectrally unlimited multiplexed imaging.