FEI MORE
LOCATION: Room 07.090
SPECIFICATIONS:
- Imaging: very fast and stable inverted widefield microscope, FRAP, TIRF
- Stand: MORE frame and enclosure
- XY-stage: motorized
- Fully equipped for live imaging, temperature and CO2 controlled
- Software: Live Acquisition 2.5
- Camera: Hamamatsu ORCA flash 4.0 cooled sCMOS
- Effective number of pixels: 2048 x 2048
- Cell size: 6.5µm x 6.5µm
- Effective Area: 13.312mm x 13.312mm
- Maximum frame rate: 30fps
- Dynamic range: 16-bit
- Quantum efficiency (peak): > 70%
- Read out noise: 1.9 electrons rms
OBJECTIVES | ||||
Objective | Magnification / NA | Medium | Contrast | Working Distance |
U Plan S Apo | 10x / 0.40 | air | PH2 | 3.1 mm |
U Plan S Apo | 40x / 0.95 | air | PH2 | 0.38 mm |
TIRF APON | 60x / 1.49 | oil | - | 0.1 mm |
U Plan S Apo | 100x / 1.4 | oil | - | 0.13 mm |
ILLUMINATION | |||
Wavelengths (nm) | Availability | Type | Some dyes and use |
405 | YES | Solid state laser | DAPI |
488 | YES | Solid state laser | GFP, Alexa488, Cy2, FITC |
561 | YES | Solid state laser | TRITC |
640 | YES | Solid state laser | DRAQ5, Cy5, Alexa647 |
395/25 | YES | Solid state light | DAPI |
434/17 | YES | Solid state light | CFP |
470/24 | YES | Solid state light | GFP, Alexa488, Cy2, FITC |
510/10 | YES | Solid state light | YFP |
550/15 | YES | Solid state light | TRITC, Cy3 |
575/25 | YES | Solid state light | On demand (mCherry) |
631/28 | YES | Solid state light | DRAQ5, Cy5, Alexa647 |
470 | YES | LED (Trans) | Transillumination |
540 | YES | LED (Trans) | Transillumination |
630 | YES | LED (Trans) | Transillumination |
BANDPASS FILTERS (Emitters) | |||
Fluorochromes | Availability | Bandpass | Transmission (nm) |
DAPI | YES | single BP | 425-443 |
GFP, Alexa488 | YES | single BP | 507-537 |
RFP, Alexa568 | YES | single BP | 665-705 |
CFP / YFP | YES | dual BP | 457-471 / 536-558 |
DAPI / GFP / Cy3 / Cy5 | YES | quad BP (LED) | 442-442 / 506-524 / 585-605 / 692-768 |
DAPI / FITC / TRITC / Cy5 | YES | qud BP (Lasers) | 370-410 / 491-552 / 590-628 / 663-703 |