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LEICA POINT SCANNING CONFOCAL “Stellaris 8 FALCON“

LOCATION: Room 07.081

SPECIFICATIONS:

  • Stellaris 8: confocal point scanning system, including:

    • Pulsed White Light Laser as excitation light source, excitation range from 440nm - 790nm.

    • Direct MODulation 405 laser (DMOD)
    • Acousto Optical Beam Splitter (AOBS serves as a tunable dichroic for all lasers)

    • Highly sensitive prism-based spectral detection design with computer controlled adjustable bandwidth for all fluorescence channels.

    • detection range up to 850 nm.
  • Resonant scanner
  • Stand: Inverted DMi8 CS Premium (fully motorized and and touch screen)
  • Leica LED 3
  • Equipped with 4 HyDs
  • Fully equipped for live imaging, temperature and CO2 controlled
  • FALCON (Fast Lifetime CONtrast) providing fast FLIM, including FLIM analysis tools, e.g. exponential fitting, FLIM phasor analysis, pattern fitting and FLIM-FRET analyzer. 
  • Software: LAS X (+ 1 offline licence)
  • 1 AIVIA licence

OBJECTIVES

Objective

Magnification / NA

Medium

Working Distance

Notes

HC PL FLUOTAR

5x/0.15

air

12.0 mm

with and without coverglass

HC PL FLUOTAR

10x/0.30

air

11.0 mm

with and without coverglass

HC PL APO

20x/0.75

air

0.62 mm

0.17 mm coverglass

HC FLUOTAR L

25x/0.95

water

2.5 mm

0.17 mm coverglass

Color corrected for VIS and NIR up to 950 nm.

HC PL APO

CORR CS2

40x/1.10

 

water

0.62 mm

Correction collar for use with 0.14-0.19 mm cover glass.

Water and saltwater immersion, Temperature: 20-40 degrees.

HC PL APO

63x/1.40

oil

0.14 mm

0.17 mm coverglass

LASER LINES
White Light Laser (WLL)
  • Spectral range from 440-790 nm
  • Pulsed
Laser 405 DMOD
  • not pulsed

FILTERCUBES

Fluorochromes

Availability

Excitation

Emission

LED 405

YES

405/60

470/40

GFP

YES

470/40

525/50

DsRed

YES

546/11

605/75

DETECTORS 

Detectors Notes

 

 

 

2 Power HyD S

  • Position 1
  • Position 2
  • Versatile detector, high performance throughout the spectrum

  • 2x increased photon detection efficiency in the blue-green range
  • Typical photon detection efficiency > 58% @ 500nm.

  • Analog, reflection and photon counting detection modes

Power HyD X

  • Position 4
  • Specialized for fast FALCON in the visible up to far-red.
  • Leica GaAsp-based technology.
  • Typical photon detection efficiency > 46% @ 500nm.

Poser HyD R SP

  • Position 5
  • Specialized for near infrared detection
  • Specialized for FALCON in near infrared range
  • Special GaAsp photocathode
  • Digital and photon counting detection modes