Nikon Ti2 "seqFISH"
LOCATION: Room 07.057
SPECIFICATIONS:
- Imaging: very flexible and stable inverted widefield microscope
- Stand: Ti2-E Inverted with perfect focus and large field of view (F-mount)
- XY-stage: fully motorized
- Fully equipped for live imaging, temperature and CO2 controlled
- Software: NIS Elements with JOBS
- Camera: Photometrics Prime 95B with large field of view of 25 mm, F-mount, 11um pixel size
- Light source: Lumencor SpectraX; Lasers for sequential FISH
OBJECTIVES | ||||
Objective | Magnification / NA | Medium | Contrast | Working Distance |
CFI Plan Apo Lamda | 10x / 0.45 | air | standard DIC | 4 mm |
CFI Plan Apo Lamda | 60x / 1.4 | oil | HighRes DIC | 0.13 mm |
CFI Plan Apo Lamda | 100x / 1.45 | oil | HighRes DIC | 0.13 mm |
Note: Extra 1.5 magnification lens available on the Nikon Ti2
ILLUMINATION | |||
Wavelengths (nm) | Availability | Type | Some dyes and use |
405 | YES | Solid state laser | DAPI |
488 | YES | Solid state laser | GFP, Alexa488, Cy2, FITC |
561 | YES | Solid state laser | TRITC, strong for bleaching |
640 | YES | Solid state laser | DRAQ5, Cy5, Alexa647 |
750 | YES | Solid state laser | Cy7, Alexa750 |
395/25 | YES | Solid state light | DAPI |
440/20 | YES | Solid state light | CFP |
470/24 | YES | Solid state light | GFP, Alexa488, Cy2 FITC |
510/25 | YES | Solid state light | YFP |
550/15 | YES | Solid state light | TRITC, Cy3 |
575/25 | YES | Solid state light | mCherry, on demand |
640/30 | YES | Solid state light | DRAQ5, Cy5, Alexa639 |
DICHROIC MIRROR 5x Penta-bandpass: LED-DA/FI/TR/Cy5/Cy7-5X-A (DAPI / FITC / TRITC / Cy5 / Cy7 - Pinkel Penta) | |||
Fluorochromes | Availability | Mirror (nm) | Transmission (nm) |
DAPI | YES | 414-450 | 420-460 |
GFP, Alexa488 | YES | 500-530 | 510-531 |
RFP, Alexa568 | YES | 580-611 | 590-624 |
Cy5, Alexa647 | YES | 661-701 | 677-712 |
Cy7, Alexa750 | YES | 769-850 | 769-850 |
EMISSION FILTERS in front of the camera | |||
Fluorochromes | Availability | Bandpass | Transmission (nm) |
DAPI | YES | single BP | 435-485 |
GFP, Alexa488 | YES | single BP | 515-555 |
RFP, Alexa568 | YES | single BP | 565-615 |
Cy5, Alexa647 | YES | single BP | peak 670 |