ZEISS Lightsheet 7
LOCATION: Room 07.092
SPECIFICATIONS:
- Versatile lightsheet microscope to image fixed and live samples
- Stand: Zeiss Z1 lightsheet microscope
- Samples mounted vertically on a motorized stage with XYZ and rotation
- Imaging chambers for water-based media (RI = 1.33) or clearing media (RI = 1.33 - 1.58)
- Fully equipped for live-imaging with temperature and CO2 control
- Light sheet thickness: ~2 µm - 14 µm
- Dual side illumination and multi-view acquisition
- Pivot scan for removing shadows
- Software: Zen Black for light sheet microscopy
CAMERA SPECIFICATIONS:
- 2 liquid cooled sCMOS cameras, pco.EDGE
- Effective number of pixels: 1920 x 1920
- Resolution: 0.23 μm pixel size
- Max frame rate: 30 frames/second
- Dynamic range of 36 000:1
- 16-bit camera readout
- extreme low readout noise of 0.8 e- med
- quantum efficiency above 70%
| LASER LINES | ||
| Fixed wavelengths (nm) | Laser type | Example dyes |
| 405 | solid state, 20 mW | DAPI, Hoechst |
| 488 | solid state, 30 mW | GFP, Alexa 488 |
| 561 | solid state, 20 mW | Alexa 568, Cy3 |
| 640 | solid state, 75 mW | Alexa 647, Cy5 |
| ILLUMINATION OBJECTIVES | |||
| Objective | Magnification / NA | Immersion medium | Combine with |
| LSFM foc | 5x / 0.1 | air | 2.5x, 5x and 10x |
| LSFM foc | 10x / 0.2 | air | 20x, 40x and 63x |
| DETECTION OBJECTIVES | ||||
| Objective | Magnification / NA | Immersion medium | Chamber (RI) | Working distance (mm) |
| Fluar | 2.5x / 0.12 | air | clearing ( > 1.46) | 8.7 |
| Plan NeoFluar | 5x / 0.16 | air | water and clearing (1.33 - 1.58) | 5.1 |
| Plan Apochromat | 10x / 0.5 | water | water (1.33) | 3.7 |
| Plan Apochromat | 20x / 1.0 | water dipping | water (1.33) | 2.4 |
| Plan Apochromat | 20x / 1.0 | clarity dipping | clarity (1.46) | 5.6 |
| Plan Apochromat | 20x / 1.0 | ECi dipping | ECi (1.48 - 1.58) | 5.6 |
| Plan Apochromat | 40x / 1.0 | water dipping | water (1.33) | 2.5 |
| Plan Apochromat | 63x / 1.0 | water dipping | water (1.33) | 2.1 |
| FILTER SETS | ||||
| Fluorochromes | Wavelengths (nm) | Mirror (nm) | Filter 1 (nm) | Filter 2 (nm) |
| Blue / Green | 405 / 488 | LP 510 | BP 420 - 470 | BP 525 - 545 |
| Green / Red | 488 / 561 | LP 560 | BP 505 - 545 | BP 575 - 615 |
| Green / Red | 488 / 561 | LP 560 | BP 505 - 545 | LP 585 |
| Green / Far Red | 488 / 638 | LP 560 | BP 505 - 545 | LP 660 |