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ZEISS POINT SCANNING CONFOCAL “LSM880 Inverted“ WITH AIRYSCAN

LOCATION: Room 07.083

SPECIFICATIONS:

  • Imaging: Confocal for live; large samples; Airyscan
  • Stand: Zeiss Axio Observer
  • XYZ-stage: motorized
  • Fully equipped for live imaging, Temperature and CO2 controlled
  • Integrated stitching tool
  • Widefield camera to easily set tile experiments
  • Airyscan FAST technique increases acquisition speed > 4x
  • Airyscan SR technique increase by 1.7 your resolution.
  • Software: Zen Blue

  

OBJECTIVES

Objective

Magnification / NA

Medium

Contrast

Working Distance

PLAN APO

10x / 0.45

air

DIC

2 mm

PLAN APO

25x / 0.80

imm

DIC

0.57 mm

PLAN APO

40x / 1.20

sil/imm

DIC

0.41 mm

PLAN APO

63x / 1.40

oil

DIC

0.19 mm

LASER LINES

Wavelengths (nm)

Availability

Type

Some dyes and use

405

YES

Solid state, 5mW

Alexa405, DAPI, Hoechst

488

YES

Solid state, 10mW

Alexa488, GFP, Cy2

555

YES

Solid state, 10mW

RedRFP, Alexa568

639

YES

Solid state, 5mW

Alexa647, Cy5, DRAQ5

FILTER CUBES

Fluorochromes

Availability

Excitation

Dichroic

Emission

Filter No

Blue

YES

G 365

FT 395

420 - 470

49 DAPI

Green

YES

450 - 490

FT 495

500 - 550

38HE eGFP

Red

YES

540 - 552

FT 560

575 - 640

20 Rhodamine