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Nikon Ti2 "seqFISH"

LOCATION: Room 07.057

SPECIFICATIONS:

  • Imaging: very flexible and stable inverted widefield microscope
  • Stand: Ti2-E Inverted with perfect focus and large field of view (F-mount)
  • XY-stage: fully motorized
  • Fully equipped for live imaging, temperature and CO2 controlled
  • Software: NIS Elements with JOBS
  • Camera: Photometrics Prime 95B with large field of view of 25 mm, F-mount, 11um pixel size
  • Light source: Lumencor SpectraX; Lasers for sequential FISH

OBJECTIVES

Objective

Magnification / NA

Medium

Contrast

Working Distance

CFI Plan Apo Lamda

10x / 0.45

air

standard DIC

4 mm

CFI Plan Apo Lamda

60x / 1.4

oil

HighRes DIC

0.13 mm

CFI Plan Apo Lamda

100x / 1.45

oil

HighRes DIC

0.13 mm

Note: Extra 1.5 magnification lens available on the Nikon Ti2

ILLUMINATION

Wavelengths (nm)

Availability

Type

Some dyes and use

405

YES

Solid state laser

DAPI

488

YES

Solid state laser

GFP, Alexa488, Cy2, FITC

561

YES

Solid state laser

TRITC, strong for bleaching

640

YES

Solid state laser

DRAQ5, Cy5, Alexa647

750

YES

Solid state laser

Cy7, Alexa750

395/25

YES

Solid state light

DAPI

440/20

YES

Solid state light

CFP

470/24

YES

Solid state light

GFP, Alexa488, Cy2 FITC

510/25

YES

Solid state light

YFP

550/15

YES

Solid state light

TRITC, Cy3

575/25

YES

Solid state light

mCherry, on demand

635/18

YES

Solid state light

DRAQ5, Cy5, Alexa639

DICHROIC MIRROR

5x Penta-bandpass: LED-DA/FI/TR/Cy5/Cy7-5X-A (DAPI / FITC / TRITC / Cy5 / Cy7 - Pinkel Penta)
FF409/493/573/652/759-Di01-25x36

Fluorochromes

Reflection to sample (nm)

Transmission to camera (nm)

DAPI

350-404

414-450

GFP, Alexa488

461-487.5

499.5-530

RFP, Alexa568

543-566

580-611

Cy5, Alexa647

626-644

661-701

Cy7, Alexa750

721-749

768.5-849.5

EMISSION FILTERS in front of the camera

Fluorochromes

Availability

Bandpass

Transmission (nm)

DAPI

YES

single BP

432/36

GFP, Alexa488

YES

single BP

515/30

RFP, Alexa568

YES

single BP

595/31

Cy5, Alexa647

YES

single BP

680/42

Cy7, Alexa750 YES single BP 809/81
penta-EM YES penta-band

FF01-432/515/595/681/809