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ZEISS lightsheet microscope “Lightsheet 7“
LOCATION: Room 07.092
SPECIFICATIONS:
- Imaging: Lightsheet for live & fixed large samples
- Stand: Zeiss Z1 lightsheet microscope
- XYZ & rotation-stage: motorized
- Fully equipped for live-imaging, Temperature and CO2 controlled
- Integrated multi-view reconstruction, deconvolution
- Software: Zen 2010 for light sheet microscopy
CAMERA SPECIFICATIONS:
- 2 liquid cooled sCMOS cameras, pco.EDGE
- Effective number of pixels: 1920 x 1920
- Resolution: 0.23 μm pixel size
- Max frame rate: 30 frames/second
- Dynamic range: 16 bit readout
- dynamic range of 36 000 : 1
- extreme low readout noise of 0.8 e- med
- quantum efficiency above 70%
OBJECTIVES | ||||
Objective | Magnification / NA | Medium | Chamber/RI | Working Distance |
FLUAR | 2.5x / 0.12 | air | clearing | - |
PLAN NeoFluar | 5x / 0.16 | air | water & clearing 1.33 - 1.58 | 5.1 mm |
PLAN APO | 20x / 1.0 | water dipping | water 1.33 | 2.4 mm |
PLAN APO | 40x / 1.0 | water dipping | water 1.33 | 2.5 mm |
PLAN APO | 63x / 1.0 | water dipping | water 1.33 | 2.1 mm |
PLAN APO | 20x / 1.0 | clarity dipping | clarity 1.46 | 5.6 mm |
PLAN APO | 20x / 1.0 | ECi dipping | ECi 1.48 - 1.58 | 5.6 mm |
LASER LINES | ||
FIXED wavelengths | Laser type | Some dyes and use |
405nm | solid state, 5mW | DAPI |
488nm | solid state, 10mW | GFP, Alexa488, Cy2 |
561nm | solid state, 10mW | RedFPs, Alexa568, Cy3 |
640nm | solid state, 5mW | DRAQ5, Cy5, Alexa647 |
FILTER SETS | ||||
Fluorochromes | Wavelengths (nm) | Mirror (nm) | Filter 1 (nm) | Filter 2 (nm) |
Blue / Green | 405 / 488 | LP 510 | 420 - 470 | 525 - 545 |
Green / Red | 488 / 561 | LP 560 | 505 - 545 | 575 - 615 |
Green / Red | 488 / 561 | LP560 | 505 - 545 | LP 585 |
Green / Far Red | 488 / 638 | LP 560 | 505 - 545 | LP 660 |