Navigation mit Access Keys

D3: Large Scale Protein Production of Functional Proteins – 17001

(2 hrs/week; 2 CP; Fall 2025)

Sebastian Hiller, Timm Maier

Large amounts of functionally and structurally intact protein are essential for structural biology and many other fields of biochemistry and biophysics. Such amounts are rarely available from native material. This lecture series will focus on the various approaches for large-scale protein production in prokaryotic and eukaryotic cell lines as well as in vitro systems, their respective advantages and disadvantages, strategies to overcome the various problems inherent to the systems and on the functional recovery of the proteins themselves.

Schedule

Date

Time


Type

Speaker

23.02.2023

13.15-15.00

On-site

Intro: Organization

02.03.2023

 

Fasnachstferien

09.03.2023

13.15-15.00

On-site

Intro: Lecture Timm Maier

16.03.2023

13.15-15.00

On-site

Gregor Weiss, ETH Zürich
Bacterial cell-cell interactions - from environmental to patient samples

23.03.2023

13.15-15.00

Zoom

Mario Halic, St Jude Children’s Research Hospital, USA
Chromatin dynamics - How do we visualize dynamic chromatin complexes?

30.03.2023

13.15-15.00

On-site

Ashwin Chari, MPI-NAT Göttingen, Germany
Structural Biochemistry of Large Macromolecular Complexes

06.04.2023

 

Ostern / Easter break

13.04.2023

13.15-15.00

Zoom
 

Moritz Hunkeler, Harvard University, USA
BIRC6: A Giant Ubiquitin Ligase Controlling Cell Death Signaling

20.04.2023

13.15-15.00

On-site

Timm Maier

27.04.2023

13.15-15.00

On-site

Patrick Fischer, Hiller lab, Biozentrum
A practical guide to molecular cloning using the in vivo assembly (IVA) method

04.05.2023

13.15-15.00

Zoom

Silvija Bilokapic St Jude Children’s Research Hospital, USA
Chromatin localization of nucleophosmin organizes ribosome biogenesis

11.05.2023

13.15-15.00

On-site

Justin Kollman, University of Washington, USA
Allosteric control of nucleotide biosynthesis by enzyme polymerization 

18.05.2023

 

Auffahrt

25.05.2023

13.15-15.00

On-site

Student Presentation

01.06.2023

13.15-15.00

On-site

Student Presentation

 

On-site / zoom meetings and student chalk talks are scheduled on Thursday 13:15 – 15:00 in room U1.193