D3: Large Scale Protein Production of Functional Proteins – 17001
(2 hrs/week; 2 CP; Fall 2025)
Sebastian Hiller, Timm Maier
Large amounts of functionally and structurally intact protein are essential for structural biology and many other fields of biochemistry and biophysics. Such amounts are rarely available from native material. This lecture series will focus on the various approaches for large-scale protein production in prokaryotic and eukaryotic cell lines as well as in vitro systems, their respective advantages and disadvantages, strategies to overcome the various problems inherent to the systems and on the functional recovery of the proteins themselves.
Schedule
Date | Time |
| Speaker |
23.02.2023 | 13.15-15.00 | On-site | Intro: Organization |
02.03.2023 |
| Fasnachstferien | |
09.03.2023 | 13.15-15.00 | On-site | Intro: Lecture Timm Maier |
16.03.2023 | 13.15-15.00 | On-site | Gregor Weiss, ETH Zürich |
23.03.2023 | 13.15-15.00 | Zoom | Mario Halic, St Jude Children’s Research Hospital, USA |
30.03.2023 | 13.15-15.00 | On-site | Ashwin Chari, MPI-NAT Göttingen, Germany |
06.04.2023 |
| Ostern / Easter break | |
13.04.2023 | 13.15-15.00 | Zoom | Moritz Hunkeler, Harvard University, USA |
20.04.2023 | 13.15-15.00 | On-site | Timm Maier |
27.04.2023 | 13.15-15.00 | On-site | Patrick Fischer, Hiller lab, Biozentrum |
04.05.2023 | 13.15-15.00 | Zoom | Silvija Bilokapic St Jude Children’s Research Hospital, USA |
11.05.2023 | 13.15-15.00 | On-site | Justin Kollman, University of Washington, USA |
18.05.2023 |
| Auffahrt | |
25.05.2023 | 13.15-15.00 | On-site | Student Presentation |
01.06.2023 | 13.15-15.00 | On-site | Student Presentation |
On-site / zoom meetings and student chalk talks are scheduled on Thursday 13:15 – 15:00 in room U1.193