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Thermo Fisher Scientific Aquilos 2 Cryo-FIB

The Aquilos 2 cryo-FIB is the latest generation cryo-DualBeam instrument with enhanced anti-contamination hardware for improved cryo-sample protection and minimal ice contamination.

Specifically designed for use within the cryo-tomography workflow. Designed to produce electron-transparent cryo-lamellas for subsequent high-resolution cryo electron microscopy.

Fitted with an integrated iFLM correlative fluorescence light microscope which allows targeting of fluorescently labelled cells for lamella production. This offers increased throughput and specificity and can reduce the number of sample transfer steps to limit ice contamination.

High-end sample preparation platform for cryo-lamella workflow production for subsequent cryo-electron microscopy imaging and tomography. Samples can be produced under optimal cryo conditions to preserve details at the molecular level in their close to native state.

Thermo Fisher Scientific Aquilos 2 Key Specifications

Electron source

High stability Schottky field emission gun, 200 V – 30 kV accelerating voltage

Minimum electron source lifetime

12 months

Ion Source

500 V to 30 kV, 1.5pA-65 nA

Ion source resolution (with cryo-stage)

7.0 nm @ 30kV

Ion source lifetime

1,300 hours

Electron optics resolution (nm)

6 nm @ 2 kV (cryo-temperature)

Vacuum system

Completely oil free

Chamber vacuum

<8xe-5 Pa