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Instruments & Services
Welcome to the Biozentrum FACS Core Facility. We provide state-of-the-art flow cytometry instruments and support the users in their analysis and sorting needs. We offer scientific advice and customized training for experimental setup, the use of our analytical flow cytometers and data analysis. Moreover we provide operator assisted cell sorting sessions.
BD FACS AriaFusion Cell Sorter
The AriaFusion is a cuvette based cell sorter, has 5 lasers installed and is capable to measure up to 18 fluorescent parameters. It is possible to perform sterile bulk sorts for up to 4 populations simultaneously or single cell sorts into 96-well, 384-well plates and other custom formats for various downstream applications.
BD FACS Melody Cell Sorter
The Melody is a cuvette based cell sorter, has 3 lasers installed and is capable to measure up to 8 fluorescent parameters. It is possible to perform aseptic bulk sorts for up to 2 populations simultaneously or single cell sorts into 96-well, 384-well plates and other custom formats for various downstream applications. It is an easy to use cell sorter and is open for unassisted sorting for Biozentrum and University members.
BD FACS CantoII Analyzer
This analyzer has 3 lasers installed and detects up to 8 colors simultaneously. It can be run with FACS tubes and has a high-throughput option for 96-well plates. The Canto is easy to use and well suited for beginners in flow cytometry.
BD LSR Fortessa Analyzer
Our Fortessa has 4 lasers installed and detects up to 16 fluorescence parameters in parallel. The samples can be measured with FACS tubes or with the high-throughput system for 96-well plates. This instrument has a very sensitive detection and the configuration is optimal for fluorescent protein and multicolorapplications.
BD FACS AriaIII Cell Sorter
The Aria is a cuvette based cell sorter, has 4 lasers installed and is capable to measure up to 13 fluorescent parameters. It is possible to perform aseptic bulk sorts for up to 4 populations simultaneously or single cell sorts into 96-well, 384-well plates and other custom formats for various downstream applications.
10x Chromium Controller
We are hosting the controller from 10x to generate the library for scRNA-Seq in our facility. Therefore we can enrich and purify the samples by cell sorting and immediately process it for sequencing. The instrument can be booked via our booking homepage PPMS.
For more information contact the FACS Core Facility and the Genomics Facility.
Education and Training
We offer hands-on instrument trainings for the independent use of our analytical flow cytometers and for the Melody cell sorter. Learn here how to schedule an instrument training. Furthermore we provide several teachings based on of flow cytometry. If you have any special training needs please contact us directly.
Data Analysis
We offer two FlowJo licenses that can be booked over our booking homepage. Additionally the users have the possibility to buy an annual FlowJo on-site license for their own use on a dedicated device. Other common software to analyze flow cytometry data include FCSExpress, Cytobank, and the freely available Flowing Software. For more information regarding analysis software and analysis assistance please contact the FACS Core Facility team.