Configuration
200kV X-FEG optics. C-TWIN objective lens. Autoloader for multi-grid loading at cryo-LN2 temperature and automated exchange of grid to column. 

The Glacios instrument is housed in a metal system enclosure which reduces its sensitivity to sound and temperature disturbances or fluctuations.

Two detectors are present; Thermo Fisher Scientific CETA 16M Pixel CMOS camera ideal for screening and setting up sessions; Gatan K3 2416M Pixel (5,760 x 4,092) direct Electron Detector .

Fitted with a Volta Phase Plate.

EPU and SerialEM software for automated EM data acquisition.

Application
The Glacios is an advanced cryo-transmission electron microscope that can used in a single particle analysis (SPA) workflow for pre-screening and assessment of sample quality before transferring to the Titan Krios cryo-TEM for ultimate-resolution SPA data acquisition.

EPU software can be used for automated atlas screening to gauge grid ice quality using the Ceta camera. Furthermore, the Glacios cryo-TEM is configured with a K3 Direct Electron Detector to be a complete standalone SPA data acquisition solution. Digital FluCam can be used for initial alignments, microscope set-up and optimization.

SerialEM software is used for longer term unattended acquisition after suitable grid selection and set-up. Electron tomography applications are also possible under SerialEM.

Specifications