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FEI Tecnai G2 Spirit TEM

BioTWIN lens configuration for increased contrast. 120kV TEM microscope (operated at 80kV) with a LaB6 filament and an EMSIS Veleta camera (top, side-mounted). The Camera operates using RADIUS software from EMSIS.

General purpose transmission electron microscope. Ideal for screening grids of negatively stained samples or thin sections at room temperature (microscope is not cryo-capable but is operated with a cryo-shield).

FEI Tecnai G2 Spirit BioTWIN Specifications 

Accelerating Voltage

80 kV nominally

Electron Source


Stage Tilt Range

± 70°

Line Resolution

0.34 nm

Cs Objective

6.3 mm

Specimen Exchange

< 5 minutes

Operating Temperature

Room temperature; approx. 21 degrees; cryo shield cooled in vacuum with LN2

Veleta CCD Camera


Retractable (pneumatic), side mounted (top) peltier cooled, lens coupled CCD camera

·         Sensor size 2048 x 2048 pixels (4 megapixels).

·         Sensor active area 16.67mm x 16.67 mm

·         Peltier cooled sensor 10°C @ 25 °C ambient temperature

·         Noise reduction – Correlated double sampling

·         Physical pixel size 7.4 µm2

·         Dynamic Range 14 bit

·         5 fps @ full resolution

Camera acquisition software